Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult rats

Daniel Becker, Devin S. Gary, Ephron S. Rosenzweig, Warren M. Grill, John W. McDonald

Research output: Contribution to journalArticle

Abstract

Functional electrical stimulation (FES) can restore control and offset atrophy to muscles after neurological injury. However, FES has not been considered as a method for enhancing CNS regeneration. This paper demonstrates that FES dramatically enhanced progenitor cell birth in the spinal cord of rats with a chronic spinal cord injury (SCI). A complete SCI at thoracic level 8/9 was performed on 12 rats. Three weeks later, a FES device to stimulate hindlimb movement was implanted into these rats. Twelve identically-injured rats received inactive FES implants. An additional control group of uninjured rats were also examined. Ten days after FES implantation, dividing cells were marked with bromodeoxyuridine (BrdU). The "cell birth" subgroup (half the animals in each group) was sacrificed immediately after completion of BrdU administration, and the "cell survival" subgroup was sacrificed 7 days later. In the injured "cell birth" subgroup, FES induced an 82-86% increase in cell birth in the lumbar spinal cord. In the injured "cell survival" subgroup, the increased lumbar newborn cell counts persisted. FES doubled the proportion of the newly-born cells which expressed nestin and other markers suggestive of tripotential progenitors. In uninjured rats, FES had no effect on cell birth/survival. This report suggests that controlled electrical activation of the CNS may enhance spontaneous regeneration after neurological injuries.

Original languageEnglish (US)
Pages (from-to)211-218
Number of pages8
JournalExperimental Neurology
Volume222
Issue number2
DOIs
StatePublished - Apr 2010

Fingerprint

Electric Stimulation
Spinal Cord
Stem Cells
Parturition
Cell Survival
Bromodeoxyuridine
Spinal Cord Injuries
Regeneration
Nestin
Muscular Atrophy
Wounds and Injuries
Hindlimb
Thorax
Cell Count
Equipment and Supplies
Control Groups

Keywords

  • Cell birth
  • Exercise
  • Neural activity
  • Regeneration
  • Rehabilitation
  • Spinal cord injury
  • Stem cell

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult rats. / Becker, Daniel; Gary, Devin S.; Rosenzweig, Ephron S.; Grill, Warren M.; McDonald, John W.

In: Experimental Neurology, Vol. 222, No. 2, 04.2010, p. 211-218.

Research output: Contribution to journalArticle

Becker, Daniel ; Gary, Devin S. ; Rosenzweig, Ephron S. ; Grill, Warren M. ; McDonald, John W. / Functional electrical stimulation helps replenish progenitor cells in the injured spinal cord of adult rats. In: Experimental Neurology. 2010 ; Vol. 222, No. 2. pp. 211-218.
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