Functional consequences of the interactions among the Neural Cell Adhesion Molecule NCAM, the receptor tyrosine kinase TrkB, and the inwardly rectifying K+ channel KIR3.3

Ralf Kleene, Claas Cassens, Robert Bähring, Thomas Theis, Meifang Xiao, Alexander Dityatev, Claus Schafer-Nielsen, Frank Döring, Erhard Wischmeyer, Melitta Schachner

Research output: Contribution to journalArticle

Abstract

Cell adhesion molecules and neurotrophin receptors are crucial for the development and the function of the nervous system. Among downstream effectors of neurotrophin receptors and recognition molecules are ion channels. Here, we provide evidence that G protein-coupled inwardly rectifying K+ channel Kir3.3 directly binds to the neural cell adhesion molecule (NCAM) and neurotrophin receptor TrkB. We identified the binding sites for NCAM and TrkB at the C-terminal intracellular domain of Kir3.3. The interaction between NCAM, TrkB, and Kir3.3 was supported by immunocytochemical co-localization of Kir3.3, NCAM, and/or TrkB at the surface of hippocampal neurons. Co-expression of TrkB and Kir3.1/3.3 in Xenopus oocytes increased the K+ currents evoked by Kir3.1/3.3 channels. This current enhancement was reduced by the concomitant co-expression with NCAM. Both surface fluorescence measurements of microinjected oocytes and cell surface biotinylation of transfected CHO cells indicated that the cell membrane localization of Kir3.3 is regulated by TrkB and NCAM. Furthermore, the level of Kir3.3, but not of Kir3.2, at the plasma membranes was reduced in TrkB-deficient mice, supporting the notion that TrkB regulates the cell surface expression of Kir3.3. The premature expression of developmentally late appearing Kir3.1/3.3 in hippocampal neurons led to a reduction of NCAM-induced neurite outgrowth. Our observations indicate a decisive role for the neuronal K+ channel in regulating NCAM-dependent neurite outgrowth and attribute a physiologically meaningful role to the functional interplay of Kir3.3, NCAM, and TrkB in ontogeny.

Original languageEnglish (US)
Pages (from-to)28968-28979
Number of pages12
JournalJournal of Biological Chemistry
Volume285
Issue number37
DOIs
StatePublished - Sep 10 2010
Externally publishedYes

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Inwardly Rectifying Potassium Channel
Neural Cell Adhesion Molecules
Receptor Protein-Tyrosine Kinases
Protein-Tyrosine Kinases
Nerve Growth Factor Receptors
Cell membranes
Neurons
Oocytes
Cell Membrane
Biotinylation
adhesion receptor
CHO Cells
Cell Adhesion Molecules
Neurology
Xenopus
Ion Channels
GTP-Binding Proteins
Nervous System
Fluorescence
Binding Sites

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Functional consequences of the interactions among the Neural Cell Adhesion Molecule NCAM, the receptor tyrosine kinase TrkB, and the inwardly rectifying K+ channel KIR3.3. / Kleene, Ralf; Cassens, Claas; Bähring, Robert; Theis, Thomas; Xiao, Meifang; Dityatev, Alexander; Schafer-Nielsen, Claus; Döring, Frank; Wischmeyer, Erhard; Schachner, Melitta.

In: Journal of Biological Chemistry, Vol. 285, No. 37, 10.09.2010, p. 28968-28979.

Research output: Contribution to journalArticle

Kleene, R, Cassens, C, Bähring, R, Theis, T, Xiao, M, Dityatev, A, Schafer-Nielsen, C, Döring, F, Wischmeyer, E & Schachner, M 2010, 'Functional consequences of the interactions among the Neural Cell Adhesion Molecule NCAM, the receptor tyrosine kinase TrkB, and the inwardly rectifying K+ channel KIR3.3', Journal of Biological Chemistry, vol. 285, no. 37, pp. 28968-28979. https://doi.org/10.1074/jbc.M110.114876
Kleene, Ralf ; Cassens, Claas ; Bähring, Robert ; Theis, Thomas ; Xiao, Meifang ; Dityatev, Alexander ; Schafer-Nielsen, Claus ; Döring, Frank ; Wischmeyer, Erhard ; Schachner, Melitta. / Functional consequences of the interactions among the Neural Cell Adhesion Molecule NCAM, the receptor tyrosine kinase TrkB, and the inwardly rectifying K+ channel KIR3.3. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 37. pp. 28968-28979.
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AU - Bähring, Robert

AU - Theis, Thomas

AU - Xiao, Meifang

AU - Dityatev, Alexander

AU - Schafer-Nielsen, Claus

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AU - Wischmeyer, Erhard

AU - Schachner, Melitta

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AB - Cell adhesion molecules and neurotrophin receptors are crucial for the development and the function of the nervous system. Among downstream effectors of neurotrophin receptors and recognition molecules are ion channels. Here, we provide evidence that G protein-coupled inwardly rectifying K+ channel Kir3.3 directly binds to the neural cell adhesion molecule (NCAM) and neurotrophin receptor TrkB. We identified the binding sites for NCAM and TrkB at the C-terminal intracellular domain of Kir3.3. The interaction between NCAM, TrkB, and Kir3.3 was supported by immunocytochemical co-localization of Kir3.3, NCAM, and/or TrkB at the surface of hippocampal neurons. Co-expression of TrkB and Kir3.1/3.3 in Xenopus oocytes increased the K+ currents evoked by Kir3.1/3.3 channels. This current enhancement was reduced by the concomitant co-expression with NCAM. Both surface fluorescence measurements of microinjected oocytes and cell surface biotinylation of transfected CHO cells indicated that the cell membrane localization of Kir3.3 is regulated by TrkB and NCAM. Furthermore, the level of Kir3.3, but not of Kir3.2, at the plasma membranes was reduced in TrkB-deficient mice, supporting the notion that TrkB regulates the cell surface expression of Kir3.3. The premature expression of developmentally late appearing Kir3.1/3.3 in hippocampal neurons led to a reduction of NCAM-induced neurite outgrowth. Our observations indicate a decisive role for the neuronal K+ channel in regulating NCAM-dependent neurite outgrowth and attribute a physiologically meaningful role to the functional interplay of Kir3.3, NCAM, and TrkB in ontogeny.

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