Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis

Elizabeth A. Winzeler; Daniel D. Shoemaker; Anna Astromoff; Hong Liang; Keith Anderson; Bruno Andre; Rhonda Bangham; Rocio Benito; Jef D. Boeke; Howard Bussey; Angela M. Chu; Carla Connelly; Karen Davis; Fred Dietrich; Sally Whelen Dow; Mohamed El Bakkoury; Françoise Foury; Stephen H. Friend; Erik Gentalen; Guri Giaever; Johannes H. Hegemann; Ted Jones; Michael Laub; Hong Liao; Nicole Liebundguth; David J. Lockhart; Anca Lucau-Danila; Marc Lussier; Nasiha M'Rabet; Patrice Menard; Michael Mittmann; Chai Pai; Corinne Rebischung; Jose L. Revuelta; Linda Riles; Christopher J. Roberts; Petra Ross-MacDonald; Bart Scherens; Michael Snyder; Sharon Sookhai-Mahadeo; Reginald K. Storms; Steeve Véronneau; Marleen Voet; Guido Volckaert; Teresa R. Ward; Robert Wysocki; Grace S. Yen; Kexin Yu; Katja Zimmermann; Peter Philippsen; Mark Johnston; Ronald W. Davis

doi:10.1126/science.285.5429.901

Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis

Elizabeth A. Winzeler, Daniel D. Shoemaker, Anna Astromoff, Hong Liang, Keith Anderson, Bruno Andre, Rhonda Bangham, Rocio Benito, Jef D. Boeke, Howard Bussey, Angela M. Chu, Carla Connelly, Karen Davis, Fred Dietrich, Sally Whelen Dow, Mohamed El Bakkoury, Françoise Foury, Stephen H. Friend, Erik Gentalen, Guri GiaeverJohannes H. Hegemann, Ted Jones, Michael Laub, Hong Liao, Nicole Liebundguth, David J. Lockhart, Anca Lucau-Danila, Marc Lussier, Nasiha M'Rabet, Patrice Menard, Michael Mittmann, Chai Pai, Corinne Rebischung, Jose L. Revuelta, Linda Riles, Christopher J. Roberts, Petra Ross-MacDonald, Bart Scherens, Michael Snyder, Sharon Sookhai-Mahadeo, Reginald K. Storms, Steeve Véronneau, Marleen Voet, Guido Volckaert, Teresa R. Ward, Robert Wysocki, Grace S. Yen, Kexin Yu, Katja Zimmermann, Peter Philippsen, Mark Johnston, Ronald W. Davis

School of Medicine

Research output: Contribution to journal › Article › peer-review

2958 Scopus citations

Abstract

The functions of many open reading frames (ORFs) identified in genome- sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.

Original language	English (US)
Pages (from-to)	901-906
Number of pages	6
Journal	Science
Volume	285
Issue number	5429
DOIs	https://doi.org/10.1126/science.285.5429.901
State	Published - Aug 6 1999

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Winzeler, E. A., Shoemaker, D. D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J. D., Bussey, H., Chu, A. M., Connelly, C., Davis, K., Dietrich, F., Dow, S. W., El Bakkoury, M., Foury, F., Friend, S. H., Gentalen, E., ... Davis, R. W. (1999). Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science, 285(5429), 901-906. https://doi.org/10.1126/science.285.5429.901

Winzeler, EA, Shoemaker, DD, Astromoff, A, Liang, H, Anderson, K, Andre, B, Bangham, R, Benito, R, Boeke, JD, Bussey, H, Chu, AM, Connelly, C, Davis, K, Dietrich, F, Dow, SW, El Bakkoury, M, Foury, F, Friend, SH, Gentalen, E, Giaever, G, Hegemann, JH, Jones, T, Laub, M, Liao, H, Liebundguth, N, Lockhart, DJ, Lucau-Danila, A, Lussier, M, M'Rabet, N, Menard, P, Mittmann, M, Pai, C, Rebischung, C, Revuelta, JL, Riles, L, Roberts, CJ, Ross-MacDonald, P, Scherens, B, Snyder, M, Sookhai-Mahadeo, S, Storms, RK, Véronneau, S, Voet, M, Volckaert, G, Ward, TR, Wysocki, R, Yen, GS, Yu, K, Zimmermann, K, Philippsen, P, Johnston, M & Davis, RW 1999, 'Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis', Science, vol. 285, no. 5429, pp. 901-906. https://doi.org/10.1126/science.285.5429.901

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abstract = "The functions of many open reading frames (ORFs) identified in genome- sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.",

author = "Winzeler, {Elizabeth A.} and Shoemaker, {Daniel D.} and Anna Astromoff and Hong Liang and Keith Anderson and Bruno Andre and Rhonda Bangham and Rocio Benito and Boeke, {Jef D.} and Howard Bussey and Chu, {Angela M.} and Carla Connelly and Karen Davis and Fred Dietrich and Dow, {Sally Whelen} and {El Bakkoury}, Mohamed and Fran{\c c}oise Foury and Friend, {Stephen H.} and Erik Gentalen and Guri Giaever and Hegemann, {Johannes H.} and Ted Jones and Michael Laub and Hong Liao and Nicole Liebundguth and Lockhart, {David J.} and Anca Lucau-Danila and Marc Lussier and Nasiha M'Rabet and Patrice Menard and Michael Mittmann and Chai Pai and Corinne Rebischung and Revuelta, {Jose L.} and Linda Riles and Roberts, {Christopher J.} and Petra Ross-MacDonald and Bart Scherens and Michael Snyder and Sharon Sookhai-Mahadeo and Storms, {Reginald K.} and Steeve V{\'e}ronneau and Marleen Voet and Guido Volckaert and Ward, {Teresa R.} and Robert Wysocki and Yen, {Grace S.} and Kexin Yu and Katja Zimmermann and Peter Philippsen and Mark Johnston and Davis, {Ronald W.}",

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AU - Winzeler, Elizabeth A.

AU - Shoemaker, Daniel D.

AU - Astromoff, Anna

AU - Liang, Hong

AU - Anderson, Keith

AU - Andre, Bruno

AU - Bangham, Rhonda

AU - Benito, Rocio

AU - Boeke, Jef D.

AU - Bussey, Howard

AU - Chu, Angela M.

AU - Connelly, Carla

AU - Davis, Karen

AU - Dietrich, Fred

AU - Dow, Sally Whelen

AU - El Bakkoury, Mohamed

AU - Foury, Françoise

AU - Friend, Stephen H.

AU - Gentalen, Erik

AU - Giaever, Guri

AU - Hegemann, Johannes H.

AU - Jones, Ted

AU - Laub, Michael

AU - Liao, Hong

AU - Liebundguth, Nicole

AU - Lockhart, David J.

AU - Lucau-Danila, Anca

AU - Lussier, Marc

AU - M'Rabet, Nasiha

AU - Menard, Patrice

AU - Mittmann, Michael

AU - Pai, Chai

AU - Rebischung, Corinne

AU - Revuelta, Jose L.

AU - Riles, Linda

AU - Roberts, Christopher J.

AU - Ross-MacDonald, Petra

AU - Scherens, Bart

AU - Snyder, Michael

AU - Sookhai-Mahadeo, Sharon

AU - Storms, Reginald K.

AU - Véronneau, Steeve

AU - Voet, Marleen

AU - Volckaert, Guido

AU - Ward, Teresa R.

AU - Wysocki, Robert

AU - Yen, Grace S.

AU - Yu, Kexin

AU - Zimmermann, Katja

AU - Philippsen, Peter

AU - Johnston, Mark

AU - Davis, Ronald W.

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Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis

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