Functional characterization and developmental regulation of mouse telomerase RNA

María A. Blasco, Walter Funk, Bryant Villeponteau, Carol W. Greider

Research output: Contribution to journalArticle

Abstract

Telomerase synthesizes telomeric DNA repeats onto chromosome ends de novo. The mouse telomerase RNA component was cloned and contained only 65 percent sequence identity with the human telomerase RNA. Alteration of the template region in vivo generated altered telomerase products. The shorter template regions of the mouse and other rodent telomerase RNAs could account for the shorter distribution of products (processivity) generated by the mouse enzyme relative to the human telomerase. Amounts of telomerase RNA increased in immortal cells derived from primary mouse fibroblasts. RNA was detected in all newborn mouse tissues tested but was decreased during postnatal development.

Original languageEnglish (US)
Pages (from-to)1267-1270
Number of pages4
JournalScience
Volume269
Issue number5228
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

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ASJC Scopus subject areas

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