Functional assessment of myoblast transplantation for cardiac repair with magnetic resonance imaging

Ewout J. Van Den Bos, Richard B. Thompson, Anja Wagner, Heiko Mahrholdt, Yoshihisa Morimoto, Louise E.J. Thomson, Lynn H. Wang, Dirk J. Duncker, Robert M. Judd, Doris A. Taylor

Research output: Contribution to journalArticle

Abstract

Background: Contraction of transplanted myoblasts and their effects on function and remodeling after myocardial infarction remain controversial. Aim: We used magnetic resonance imaging (MRI) to study wall thickening and left ventricular (LV) function and geometry after myoblast transplantation. Methods and results: Three weeks after cryo-infarction rabbits were randomized to receive an injection of ∼2×108 myoblasts (n=8) or medium (n=9) into the scar. Cine MRI and contrast enhanced (ce) MRI images were acquired before injection (baseline) and 4 weeks later (endpoint). Regional wall thickening was measured at the site of transmural hyperenhancement. In the control group, regional wall thickening decreased to -15.3±8.6% at baseline, which further decreased to -18.3±5.7% at endpoint. Further, end-diastolic volume increased from 3.96±0.27 to 5.00±0.46 ml and end-systolic volume from 2.23±0.19 to 2.96±0.30 ml (both P<0.05 vs. baseline), which was accompanied by increased LV wall volumes (P<0.05 vs. baseline). In contrast, myoblast transplantation increased regional wall thickening from -11.9±15.9% at baseline to 26.9±17.0% (P<0.05 vs. control), which resulted in significantly improved two-dimensional ejection fractions at the infarct level and prevented the increase in end-diastolic and end-systolic volumes and wall volume. Conclusion: Intracardiac myoblast transplantation after myocardial infarction improves regional wall thickening and prevents progressive left ventricular remodeling.

Original languageEnglish (US)
Pages (from-to)435-443
Number of pages9
JournalEuropean Journal of Heart Failure
Volume7
Issue number4
DOIs
StatePublished - Jun 2005

Keywords

  • Cellular cardiomyoplasty
  • Magnetic resonance imaging
  • Myoblasts
  • Myocardial infarction
  • Stem cells
  • Wall thickening

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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    Van Den Bos, E. J., Thompson, R. B., Wagner, A., Mahrholdt, H., Morimoto, Y., Thomson, L. E. J., Wang, L. H., Duncker, D. J., Judd, R. M., & Taylor, D. A. (2005). Functional assessment of myoblast transplantation for cardiac repair with magnetic resonance imaging. European Journal of Heart Failure, 7(4), 435-443. https://doi.org/10.1016/j.ejheart.2003.12.022