TY - JOUR
T1 - Functional assays for analysis of yeast ste6 mutants
AU - Nijbroek, Gaby L.
AU - Michaelis, Susan
N1 - Funding Information:
Work in the laboratory on oxyanion resistance is supported by the Medical Research Council. MO is supported by a FRSQ scholarship and a Burroughs Wellcome Fund New Investigator Award in molecular parasitology, BP by an MRC scholarship, and KG and DL by FCAR studentships.
Funding Information:
Many past and present members of our laboratory contributed to the development of these protocols. We are grateful to Walter Schmidt, Amy Tam, and Diego Loayza for comments on this manuscript, and to Carol Berkower and Amy Tam who generated the a-factor immunoprecipitation and sac6 immunofluorescence data, shown in Figs. 3 and 5, respectively. This work was supported by grants GM51508 and DK48977 from the National Institutes of Health to S. M. and by a Predoctoral Minority Fellowship from the NIGMS to G. L. N.
PY - 1998
Y1 - 1998
N2 - In summary, several assays can be used to indirectly measure Ste6p function. These are the mating assay, the halo assay, and a-factor immunoprecipitation. These assays can be informative about the extent of a ste6 defect. The mating assay is the assay of choice because it is technically easy and particularly useful for assaying the complementation of the Δste6 defect by other ABC transporters. The immunoprecipitation of a- factor is useful for correlating the amount of mating with the amount of a- factor exported. However, this assay is technically more difficult and labor- intensive than the mating assay. Finally, the halo assay is useful in combination with the mating assay to identify gross transport defects.
AB - In summary, several assays can be used to indirectly measure Ste6p function. These are the mating assay, the halo assay, and a-factor immunoprecipitation. These assays can be informative about the extent of a ste6 defect. The mating assay is the assay of choice because it is technically easy and particularly useful for assaying the complementation of the Δste6 defect by other ABC transporters. The immunoprecipitation of a- factor is useful for correlating the amount of mating with the amount of a- factor exported. However, this assay is technically more difficult and labor- intensive than the mating assay. Finally, the halo assay is useful in combination with the mating assay to identify gross transport defects.
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U2 - 10.1016/S0076-6879(98)92016-X
DO - 10.1016/S0076-6879(98)92016-X
M3 - Article
C2 - 9711555
AN - SCOPUS:0032322785
SN - 0076-6879
VL - 292
SP - 193
EP - 212
JO - Methods in enzymology
JF - Methods in enzymology
ER -