Functional assays for analysis of yeast ste6 mutants

Gaby L. Nijbroek, Susan Michaelis

Research output: Contribution to journalArticlepeer-review

Abstract

In summary, several assays can be used to indirectly measure Ste6p function. These are the mating assay, the halo assay, and a-factor immunoprecipitation. These assays can be informative about the extent of a ste6 defect. The mating assay is the assay of choice because it is technically easy and particularly useful for assaying the complementation of the Δste6 defect by other ABC transporters. The immunoprecipitation of a- factor is useful for correlating the amount of mating with the amount of a- factor exported. However, this assay is technically more difficult and labor- intensive than the mating assay. Finally, the halo assay is useful in combination with the mating assay to identify gross transport defects.

Original languageEnglish (US)
Pages (from-to)193-212
Number of pages20
JournalMethods in enzymology
Volume292
DOIs
StatePublished - 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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