Functional and biochemical modifications in skeletal muscles from malarial mice

Marco A P Brotto, Mauro T. Marrelli, Leticia S. Brotto, Marcelo Jacobs-Lorena, Thomas M. Nosek

Research output: Contribution to journalArticle

Abstract

Although it is well established that patients suffering from malaria experience skeletal muscle problems (contracture, aches, fatigue, weakness), detailed studies have not been performed to investigate changes in the contractile function and biochemical properties of intact and skinned skeletal muscles of mammals infected with malaria. To this end, we investigated such features in the extensor digitorium longus (EDL, fast-twitch, glyocolytic) and in the soleus (SOL, slow-twitch, oxidative) muscles from mice infected with Plasmodium berghei. We first studied maximal tetanic force (Tmax) produced by intact control and malaria-infected muscles before, during and after fatigue. Triton-skinned muscle fibres were isolated from these muscles and used to determine isometric contractile features as well as a basic biochemical profile as analysed by silver-enhanced SDS-PAGE. We found that the T max of intact muscles and the maximal Ca2+-activated force (Fmax) of Triton-skinned muscle fibres were reduced by ∼50% in malarial muscles. In addition, the contractile proteins of Triton-skinned muscle fibres from malarial muscles were significantly less sensitive to Ca 2+. Biochemical analysis revealed that there was a significant loss of essential contractile proteins (e.g. troponins and myosin) in Triton-skinned muscle fibres from malarial muscles as compared to controls. The biochemical alterations (i.e., reduction of essential contractile proteins) seem to explain well the functional modifications resolved in both intact muscles and Triton-skinned muscle fibres and may provide a suitable paradigm for the aetiology of muscle symptoms associated with malaria.

Original languageEnglish (US)
Pages (from-to)417-425
Number of pages9
JournalExperimental Physiology
Volume90
Issue number3
DOIs
StatePublished - May 2005

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Skeletal Muscle
Muscles
Contractile Proteins
Malaria
Fatigue
Plasmodium berghei
Troponin
Contracture
Myosins
Silver
Polyacrylamide Gel Electrophoresis
Mammals
Pain

ASJC Scopus subject areas

  • Physiology

Cite this

Functional and biochemical modifications in skeletal muscles from malarial mice. / Brotto, Marco A P; Marrelli, Mauro T.; Brotto, Leticia S.; Jacobs-Lorena, Marcelo; Nosek, Thomas M.

In: Experimental Physiology, Vol. 90, No. 3, 05.2005, p. 417-425.

Research output: Contribution to journalArticle

Brotto, Marco A P ; Marrelli, Mauro T. ; Brotto, Leticia S. ; Jacobs-Lorena, Marcelo ; Nosek, Thomas M. / Functional and biochemical modifications in skeletal muscles from malarial mice. In: Experimental Physiology. 2005 ; Vol. 90, No. 3. pp. 417-425.
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