Functional analysis of specific promoter using vectors harboring GFP/RFP double fluorescent marker genes

Tao Yin, Qiaoping Qin, Shanglong Zhang, Jingmei Liu, Daming Chen

Research output: Contribution to journalArticlepeer-review

Abstract

Most studies related to determining the expression profile of genes and specific promoters used histochemical localization of the reporter gene, gusA. While the histochemical method for visualizing gusA expression suffers from several limitations in the determination of gene expression and location, especially in the tissues with high background acitivty. To solve this problem, a transient expession vector pBI221-RFP/GFP, was constructed using GFP and RFP as double fluorescent marker genes. This vector used CaMV 35S promoter to drive GFP and determine the transforming efficiency. It analyzed expression profile of the target gene and promoter through the RFP activities of the tranformed tissues. Through the specific promoter AGPL1 from watermelon and E8 promoter from tomato, it is resistible to use this vector to study the expression patterns of promoters. Results indicated that the pBI221-RFP/GFP is a very efficient transient expression vector that can be verify the functions of the genes and promoters.

Original languageEnglish (US)
Pages (from-to)2106-2110
Number of pages5
JournalShengwu Gongcheng Xuebao/Chinese Journal of Biotechnology
Volume24
Issue number12
StatePublished - Dec 1 2008

Keywords

  • Green fluorescent proteins
  • Red fluorescent proteins
  • Specific promoter
  • Transient expression vectors

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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