Functional analysis of a carboxyl-terminal phosphorylation mutant of the bovine papillomavirus E1 protein

Michael Lentz, Thomas Zanardi, Robyn Filzen, Jena Miller, Maria Hella

Research output: Contribution to journalArticle

Abstract

The papillomavirus E1 protein is essential for viral DNA replication, and phosphorylation of E1 appears to regulate protein function and DNA replication. Serine 584 of bovine papillomavirus E1 is in a conserved motif resembling a CK2 consensus site, and is phosphorylated by CK2 in vitro. Mutation of serine 584 to alanine eliminates replication of the viral genome in transient replication assays. Wild-type and mutant E1 proteins were expressed from recombinant baculoviruses and used to assess biochemical functions of the amino acid 584 substitution. Helicase enzyme activity, E1 binding to the viral E2 protein and to cellular DNA polymerase alpha-primase were all unaffected in the mutant protein. Binding of E1 to viral replication origin DNA sequences was reduced in the mutant, but not eliminated. The carboxyl-terminal region of the protein appears to play a role in regulating E1 function, and adds to a complex picture emerging for papillomavirus DNA replication control.

Original languageEnglish (US)
Pages (from-to)599-609
Number of pages11
JournalJournal of Molecular Biology
Volume316
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

DNA Replication
Phosphorylation
Mutant Proteins
Serine
Proteins
Replication Origin
Baculoviridae
Viral Genome
Viral DNA
Viral Proteins
Amino Acid Substitution
Alanine
Mutation
Enzymes
Bovine papillomavirus E1 protein

Keywords

  • DNA replication
  • E1 protein
  • Helicase
  • Papillomavirus
  • Phosphorylation

ASJC Scopus subject areas

  • Virology

Cite this

Functional analysis of a carboxyl-terminal phosphorylation mutant of the bovine papillomavirus E1 protein. / Lentz, Michael; Zanardi, Thomas; Filzen, Robyn; Miller, Jena; Hella, Maria.

In: Journal of Molecular Biology, Vol. 316, No. 3, 2002, p. 599-609.

Research output: Contribution to journalArticle

Lentz, Michael ; Zanardi, Thomas ; Filzen, Robyn ; Miller, Jena ; Hella, Maria. / Functional analysis of a carboxyl-terminal phosphorylation mutant of the bovine papillomavirus E1 protein. In: Journal of Molecular Biology. 2002 ; Vol. 316, No. 3. pp. 599-609.
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