Function of the Drosophila receptor guanylyl cyclase Gyc76C in PlexA-mediated motor axon guidance

Kayam Chak, Alex L. Kolodkin

Research output: Contribution to journalArticlepeer-review

Abstract

The second messengers cAMP and cGMP modulate attraction and repulsion mediated by neuronal guidance cues. We find that the Drosophila receptor guanylyl cyclase Gyc76C genetically interacts with Semaphorin 1a (Sema-1a) and physically associates with the Sema-1a receptor plexin A (PlexA). PlexA regulates Gyc76C catalytic activity in vitro, and each distinct Gyc76C protein domain is crucial for regulating Gyc76C activity in vitro and motor axon guidance in vivo. The cytosolic protein dGIPC interacts with Gyc76C and facilitates Sema-1a-PlexA/Gyc76C-mediated motor axon guidance. These findings provide an in vivo link between semaphorin-mediated repulsive axon guidance and alteration of intracellular neuronal cGMP levels.

Original languageEnglish (US)
Pages (from-to)136-147
Number of pages12
JournalDevelopment (Cambridge)
Volume141
Issue number1
DOIs
StatePublished - Jan 1 2014

Keywords

  • Axon guidance
  • Gyc76C
  • Plexin A
  • Receptor guanylyl cyclase
  • Semaphorin-1a
  • cGMP
  • dGIPC

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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