Function of rhodopsin in temperature discrimination in Drosophila

Wei L. Shen, Young Kwon, Abidemi A. Adegbola, Junjie Luo, Andrew Chess, Craig Montell

Research output: Contribution to journalArticle

Abstract

Many animals, including the fruit fly, are sensitive to small differences in ambient temperature. The ability of Drosophila larvae to choose their ideal temperature (18°C) over other comfortable temperatures (19° to 24°C) depends on a thermosensory signaling pathway that includes a heterotrimeric guanine nucleotide-binding protein (G protein), a phospholipase C, and the transient receptor potential TRPA1 channel. We report that mutation of the gene (ninaE) encoding a classical G protein-coupled receptor (GPCR), Drosophila rhodopsin, eliminates thermotactic discrimination in the comfortable temperature range. This role for rhodopsin in thermotaxis toward 18°C was light-independent. Introduction of mouse melanopsin restored normal thermotactic behavior in ninaE mutant larvae. We propose that rhodopsins represent a class of evolutionarily conserved GPCRs that are required for initiating thermosensory signaling cascades.

Original languageEnglish (US)
Pages (from-to)1333-1336
Number of pages4
JournalScience
Volume331
Issue number6022
DOIs
StatePublished - Mar 11 2011

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Rhodopsin
Drosophila
Temperature
Larva
Transient Receptor Potential Channels
Aptitude
Guanine Nucleotides
Type C Phospholipases
G-Protein-Coupled Receptors
GTP-Binding Proteins
Diptera
Fruit
Carrier Proteins
Light
Mutation
Genes

ASJC Scopus subject areas

  • General

Cite this

Shen, W. L., Kwon, Y., Adegbola, A. A., Luo, J., Chess, A., & Montell, C. (2011). Function of rhodopsin in temperature discrimination in Drosophila. Science, 331(6022), 1333-1336. https://doi.org/10.1126/science.1198904

Function of rhodopsin in temperature discrimination in Drosophila. / Shen, Wei L.; Kwon, Young; Adegbola, Abidemi A.; Luo, Junjie; Chess, Andrew; Montell, Craig.

In: Science, Vol. 331, No. 6022, 11.03.2011, p. 1333-1336.

Research output: Contribution to journalArticle

Shen, WL, Kwon, Y, Adegbola, AA, Luo, J, Chess, A & Montell, C 2011, 'Function of rhodopsin in temperature discrimination in Drosophila', Science, vol. 331, no. 6022, pp. 1333-1336. https://doi.org/10.1126/science.1198904
Shen WL, Kwon Y, Adegbola AA, Luo J, Chess A, Montell C. Function of rhodopsin in temperature discrimination in Drosophila. Science. 2011 Mar 11;331(6022):1333-1336. https://doi.org/10.1126/science.1198904
Shen, Wei L. ; Kwon, Young ; Adegbola, Abidemi A. ; Luo, Junjie ; Chess, Andrew ; Montell, Craig. / Function of rhodopsin in temperature discrimination in Drosophila. In: Science. 2011 ; Vol. 331, No. 6022. pp. 1333-1336.
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