Fully quantified spectral imaging reveals in vivo membrane protein interactions

Christopher King, Michael Stoneman, Valerica Raicu, Kalina Hristova

Research output: Contribution to journalArticle

Abstract

Here we introduce the fully quantified spectral imaging (FSI) method as a new tool to probe the stoichiometry and stability of protein complexes in biological membranes. The FSI method yields two dimensional membrane concentrations and FRET efficiencies in native plasma membranes. It can be used to characterize the association of membrane proteins: to differentiate between monomers, dimers, or oligomers, to produce binding (association) curves, and to measure the free energies of association in the membrane. We use the FSI method to study the lateral interactions of Vascular Endothelial Growth Factor Receptor 2 (VEGFR2), a member of the receptor tyrosine kinase (RTK) superfamily, in plasma membranes, in vivo. The knowledge gained through the use of the new method challenges the current understanding of VEGFR2 signaling.

Original languageEnglish (US)
Pages (from-to)216-229
Number of pages14
JournalIntegrative Biology (United Kingdom)
Volume8
Issue number2
DOIs
StatePublished - Feb 2016

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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