TY - GEN
T1 - Fully integraed droplet based point-of-care platform for molecular detection from crude biosamples
AU - Zhang, Yi
AU - Park, Seungkyung
AU - Yang, Samuel
AU - Wang, Tza Huei
PY - 2011/9/1
Y1 - 2011/9/1
N2 - This paper reports a droplet microfluidic, sample-to-answer platform for the detection of disease biomarkers and infectious pathogens using crude biosamples. The platform exploited the dual functionality of silica superparamagnetic particles (SSP) for solid phase extraction of DNA and magnetic actuation. This enabled the integration of sample preparation and genetic analysis within discrete droplets, including the steps of cell lysis, DNA binding, washing, elution, amplification and detection. The microfluidic device was self contained, with all reagents stored in droplets, thereby eliminating the need for fluidic coupling to external reagent reservoirs. The device incorporated unique surface topographic features to assist droplet manipulation. Pairs of micro-elevations are created to form slits that facilitate efficient splitting of SSP from droplets. In addition, a compact sample handling stage, which integrated the magnet manipulator, the droplet microfluidic device and a Peltier thermal cycler, was built for convenient droplet manipulation and real-time detection. Feasibility of the platform was demonstrated by analysing ovarian cancer biomarker Rsf-1 and detecting Escherichia coli with the real time polymerase chain reaction and the real time helicase dependent amplification.
AB - This paper reports a droplet microfluidic, sample-to-answer platform for the detection of disease biomarkers and infectious pathogens using crude biosamples. The platform exploited the dual functionality of silica superparamagnetic particles (SSP) for solid phase extraction of DNA and magnetic actuation. This enabled the integration of sample preparation and genetic analysis within discrete droplets, including the steps of cell lysis, DNA binding, washing, elution, amplification and detection. The microfluidic device was self contained, with all reagents stored in droplets, thereby eliminating the need for fluidic coupling to external reagent reservoirs. The device incorporated unique surface topographic features to assist droplet manipulation. Pairs of micro-elevations are created to form slits that facilitate efficient splitting of SSP from droplets. In addition, a compact sample handling stage, which integrated the magnet manipulator, the droplet microfluidic device and a Peltier thermal cycler, was built for convenient droplet manipulation and real-time detection. Feasibility of the platform was demonstrated by analysing ovarian cancer biomarker Rsf-1 and detecting Escherichia coli with the real time polymerase chain reaction and the real time helicase dependent amplification.
KW - Droplet
KW - PCR
KW - TAS
KW - melting curve analysis
KW - point of care
KW - real time
KW - sample preparation
UR - http://www.scopus.com/inward/record.url?scp=80052110074&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052110074&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2011.5969368
DO - 10.1109/TRANSDUCERS.2011.5969368
M3 - Conference contribution
AN - SCOPUS:80052110074
SN - 9781457701573
T3 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
SP - 1927
EP - 1930
BT - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
T2 - 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Y2 - 5 June 2011 through 9 June 2011
ER -