Full-length human immunodeficiency virus type 1 genomes from subtype C- infected seroconverters in India, with evidence of intersubtype recombination

Kavita S. Lole, Robert C Bollinger, Ramesh S. Paranjape, Deepak Gadkari, Smita S. Kulkarni, Nicole G. Novak, Roxann Ingersoll, Haynes W. Sheppard, Stuart Campbell Ray

Research output: Contribution to journalArticle

Abstract

The development of an effective human immunodeficiency virus type 1 (HIV-1) vaccine is likely to depend on knowledge of circulating variants of genes other than the commonly sequenced gag and env genes. In addition, full- genome data are particularly limited for HIV-1 subtype C, currently the most commonly transmitted subtype in India and worldwide. Likewise, little is known about sequence variation of HIV-1 in India, the country facing the largest burden of HIV worldwide. Therefore, the objective of this study was to clone and characterize the complete genome of HIV-1 from seroconverters infected with subtype C variants in India. Cocultured HIV-1 isolates were obtained from six seroincident individuals from Pune, India, and virtually full-length HIV-1 genomes were amplified, cloned, and sequenced from each. Sequence analysis revealed that five of the six genomes were of subtype C, while one was a mosaic of subtypes A and C, with multiple breakpoints in env, nef, and the 3' long terminal repeat as determined by both maximal χ2 analysis and phylogenetic bootstrapping. Sequences were compared for preservation of known cytotoxic T lymphocyte (CTL) epitopes. Compared with those of the HIV-1(LAI) sequence, 38% of well-defined CTL epitopes were identical. The proportion of nonconservative substitutions for Env, at 61%, was higher (P <0.001) than those for Gag (24%), Pol (18%), and Nef (32%). Therefore, characterized CTL epitopes demonstrated substantial differences from subtype B laboratory strains, which were most pronounced in Env. Because these clones were obtained from Indian seroconverters, they are likely to facilitate vaccine-related efforts in India by providing potential antigens for vaccine candidates as well as for assays of vaccine responsiveness.

Original languageEnglish (US)
Pages (from-to)152-160
Number of pages9
JournalJournal of Virology
Volume73
Issue number1
StatePublished - 1999

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Human immunodeficiency virus 1
Genetic Recombination
HIV-1
India
Genome
genome
cytotoxic T-lymphocytes
T-Lymphocyte Epitopes
Cytotoxic T-Lymphocytes
Vaccines
vaccines
epitopes
Clone Cells
gag Genes
clones
env Genes
terminal repeat sequences
Terminal Repeat Sequences
Sequence Analysis
genes

ASJC Scopus subject areas

  • Immunology

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Full-length human immunodeficiency virus type 1 genomes from subtype C- infected seroconverters in India, with evidence of intersubtype recombination. / Lole, Kavita S.; Bollinger, Robert C; Paranjape, Ramesh S.; Gadkari, Deepak; Kulkarni, Smita S.; Novak, Nicole G.; Ingersoll, Roxann; Sheppard, Haynes W.; Ray, Stuart Campbell.

In: Journal of Virology, Vol. 73, No. 1, 1999, p. 152-160.

Research output: Contribution to journalArticle

Lole, KS, Bollinger, RC, Paranjape, RS, Gadkari, D, Kulkarni, SS, Novak, NG, Ingersoll, R, Sheppard, HW & Ray, SC 1999, 'Full-length human immunodeficiency virus type 1 genomes from subtype C- infected seroconverters in India, with evidence of intersubtype recombination', Journal of Virology, vol. 73, no. 1, pp. 152-160.
Lole, Kavita S. ; Bollinger, Robert C ; Paranjape, Ramesh S. ; Gadkari, Deepak ; Kulkarni, Smita S. ; Novak, Nicole G. ; Ingersoll, Roxann ; Sheppard, Haynes W. ; Ray, Stuart Campbell. / Full-length human immunodeficiency virus type 1 genomes from subtype C- infected seroconverters in India, with evidence of intersubtype recombination. In: Journal of Virology. 1999 ; Vol. 73, No. 1. pp. 152-160.
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AU - Gadkari, Deepak

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AU - Ray, Stuart Campbell

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