Frontal dopaminergic abnormality in Tourette syndrome: A postmortem analysis

Dustin Y. Yoon, Colin D. Gause, James F. Leckman, Harvey S. Singer

Research output: Contribution to journalArticlepeer-review

Abstract

Frontal-subcortical abnormalities have been implicated in the pathophysiology of Tourette syndrome (TS). The goal of this study was to more extensively evaluate a possible underlying neurochemical abnormality in frontal cortex. Postmortem brain tissue from frontal and occipital regions (Brodmann's areas 4, 6, 9, 10, 11, 12, and 17) from three TS patients and three age-and sex-matched controls were analyzed by semiquantitative immunoblotting. Relative densities were measured for a variety of neurochemical markers including dopamine (D1, D2), serotonin (5HT-1A), and alpha-adrenergic (α-2A) receptors, the dopamine transporter (DAT), a monoamine terminal marker (vesicular monoamine transporter type 2, VMAT-2), and vesicular docking and release proteins (VAMP-2, synaptotagmin, SNAP-25, syntaxin, synaptophysin). Data from each TS sample, corrected for actin content, was expressed as a percentage value of its control. Results identified consistent increases of DAT and D2 receptor density in five of six frontal regions in all three TS subjects. D1 and α-2A receptor density were increased in a few frontal regions. These results support the hypothesis of a dopaminergic dysfunction in the frontal lobe and a likely role in the pathophysiology of TS.

Original languageEnglish (US)
Pages (from-to)50-56
Number of pages7
JournalJournal of the Neurological Sciences
Volume255
Issue number1-2
DOIs
StatePublished - Apr 15 2007

Keywords

  • Dopamine
  • Postmortem
  • Semiquantitative immunoblotting
  • Tourette syndrome

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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