TY - JOUR
T1 - From structure to mechanism
T2 - Electron crystallographic studies of bacteriorhodopsin
AU - Subramaniam, Sriram
AU - Hirai, Teruhisa
AU - Henderson, Richard
PY - 2002/5/15
Y1 - 2002/5/15
N2 - Bacteriorhodopsin is a protein found in cell membranes of the organism H. salinarum, where it functions as an efficient light-driven proton pump. Because bacteriorhodopsin is one of the simplest ion pumps known in biology, it has been the subject of intensive investigations over the last three decades, using methods spanning the range from femtosecond spectroscopy and crystallography to biochemistry and molecular biology. Here, we focus on the structural basis for the function of this protein, with primary emphasis on the contributions of electron microscopy and crystallography towards unravelling the mechanism of vectorial proton pumping.
AB - Bacteriorhodopsin is a protein found in cell membranes of the organism H. salinarum, where it functions as an efficient light-driven proton pump. Because bacteriorhodopsin is one of the simplest ion pumps known in biology, it has been the subject of intensive investigations over the last three decades, using methods spanning the range from femtosecond spectroscopy and crystallography to biochemistry and molecular biology. Here, we focus on the structural basis for the function of this protein, with primary emphasis on the contributions of electron microscopy and crystallography towards unravelling the mechanism of vectorial proton pumping.
KW - Conformational change
KW - Electron microscopy
KW - Image analysis
KW - Proton pump
KW - Two-dimensional crystal
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U2 - 10.1098/rsta.2001.0971
DO - 10.1098/rsta.2001.0971
M3 - Article
C2 - 12804283
AN - SCOPUS:0037093403
SN - 1364-503X
VL - 360
SP - 859
EP - 874
JO - Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences
JF - Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences
IS - 1794
ER -