From microbial genomics to meta-genomics

Antonello Covacci, Giulia C. Kennedy, Brendan Cormack, Rino Rappuoli, Stanley Falkow

Research output: Contribution to journalReview articlepeer-review

Abstract

Modern microbial genomics is best exemplified by determination of the complete nucleotide sequences of whole genomes. To date, the sequences of at least 11 nuclear genomes have been completed, with many more in progress. Most are microbial, with exceptions such as the eukaryotic genome Saccharomyces cerevisiae. Genomics, while built upon foundations laid in the era of classical genetics, has grown by leaps and bounds because of three significant technological advances: 1) high-throughput sequencing and automation technology, 2) sophisticated bioinformatics and sequence analysis tools, and 3) microarray or 'chip' technology for expression, resequencing, and polymorphism analysis. These technological advances have made it possible to generate massive amounts of nucleotide sequence data from a series of random, overlapping, and redundant clones and to assemble these data into contiguous sequences, with the final goal of elucidating complete gene structure at single nucleotide resolution. In the immediate future, linear genomics will be replaced by functional genomics and pathogens compared on an evolutionary scale.

Original languageEnglish (US)
Pages (from-to)180-192
Number of pages13
JournalDrug Development Research
Volume41
Issue number3-4
DOIs
StatePublished - Jul 1997
Externally publishedYes

Keywords

  • Differential fluorescence induction (DFI)
  • In vitro transposition
  • In vivo expression technology (IVET)
  • Microchips
  • Pathogenicity islands
  • Representational difference analysis (RDA)
  • Signature-tagged transposon method (STM)
  • Two dimensional immobilin gel electrophoresis (2DIGE)
  • Virulence genes

ASJC Scopus subject areas

  • Drug Discovery

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