Frog ventricle: Participation of SR in excitation-contraction coupling

Mark Anderson, I. J. Fox, C. R. Swayze, S. K. Donaldson

Research output: Contribution to journalArticle

Abstract

Activation of the first beat (B1) following a 60-s pause is diminished in isometrically contracting frog ventricular strips, in contrast to the augmentation documented for sarcoplasmic reticulum (SR)-dependent mammalian myocardium. However, treatment of frog ventricular strips with ouabain, an indirect inhibitor of the sarcolemmal Na+-Ca2+ exchanger, selectively enhanced postpause beats suggesting that in the absence of ouabain significant extrusion of cellular Ca2+ occurred during the pause. Because resting tension did not increase during the pause in ouabain-treated strips, the nonextruded Ca2+ must have been sequestered into a compartment such as SR. Steady-state beats were not affected by ouabain; its actions appeared to be separate from its known positive inotropism. Caffeine, a direct SR stimulus, initially enhanced B1 and subsequently decreased activation of all beats, which was consistent with initial augmentation of SR Ca2+ release and subsequent depletion of SR Ca2+ stores. Ouabain both potentiated the stimulatory effects and blocked the inhibitory effects of caffeine, suggesting that ouabain increased Ca2+ stores in the same intracellular Ca2+ pool as that acted on by caffeine, the SR. Ryanodine, an inhibitor of SR in mammalian myocardium, did not affect activation of frog myocardium. SR may be an important site for activator Ca2+ cycling in frog myocardium under control conditions as well as after long diastolic intervals in the presence of ouabain.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume256
Issue number5
StatePublished - Jan 1 1989
Externally publishedYes

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Excitation Contraction Coupling
Sarcoplasmic Reticulum
Anura
Ouabain
Myocardium
Caffeine
Ryanodine
Muscle Contraction

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Frog ventricle : Participation of SR in excitation-contraction coupling. / Anderson, Mark; Fox, I. J.; Swayze, C. R.; Donaldson, S. K.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 256, No. 5, 01.01.1989.

Research output: Contribution to journalArticle

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