Frequent Loss of Heterozygosity on Chromosome 9 in Adenocarcinoma and Squamous Cell Carcinoma of the Esophagus

Lila Tannin, Jing Yin, Xiaoling Zhou, Hiroyuki Suzuki, Hai Yan Jiang, Mun Gan Rhyu, John M. Abraham, Stephen J. Meltzer

Research output: Contribution to journalArticlepeer-review

72 Scopus citations


Loss of heterozygosity (LOH) affecting chromosome 9p has been shown to occur frequently in head and neck cancer, glioma, mesothelioma, melanoma, lung cancer, and numerous other tumor types. Chromosome 9p is therefore presumed to contain a tumor suppressor gene or genes. Since esophageal cancer shares characteristics with some of the above tumor types, we performed a detailed examination of 60 patients with squamous cell carcinoma or adenocarcinoma of the esophagus for LOH at loci D9S162, IFNA, D9S171, D9S126, D9S104, D9S165, and D9S163. Multiplex polymerase chain reactions were performed with the inclusion of one radiolabeled nucleotide, and products were electrophoresed on denaturing polyacrylamide gels. Thirty-six of the 60 patients (60%) exhibited LOH at one or more loci on chromosome 9p. Eight of 17 patients (47%) with adenocarcinoma manifested LOH, while 28 of 43 (65%) with squamous cell carcinoma showed LOH. LOH was most frequent at loci D9S171 (19 of 23, or 83%) and D9S165 (24 of 32, or 75%). These data support the hypothesis that a tumor suppressor gene or genes located on this portion of chromosome 9p exert(s) an effect on esophageal cancer development.

Original languageEnglish (US)
Pages (from-to)6094-6096
Number of pages3
JournalCancer Research
Issue number23
StatePublished - Dec 1994
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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