Abstract
Loss of heterozygosity (LOH) affecting chromosome 9p has been shown to occur frequently in head and neck cancer, glioma, mesothelioma, melanoma, lung cancer, and numerous other tumor types. Chromosome 9p is therefore presumed to contain a tumor suppressor gene or genes. Since esophageal cancer shares characteristics with some of the above tumor types, we performed a detailed examination of 60 patients with squamous cell carcinoma or adenocarcinoma of the esophagus for LOH at loci D9S162, IFNA, D9S171, D9S126, D9S104, D9S165, and D9S163. Multiplex polymerase chain reactions were performed with the inclusion of one radiolabeled nucleotide, and products were electrophoresed on denaturing polyacrylamide gels. Thirty-six of the 60 patients (60%) exhibited LOH at one or more loci on chromosome 9p. Eight of 17 patients (47%) with adenocarcinoma manifested LOH, while 28 of 43 (65%) with squamous cell carcinoma showed LOH. LOH was most frequent at loci D9S171 (19 of 23, or 83%) and D9S165 (24 of 32, or 75%). These data support the hypothesis that a tumor suppressor gene or genes located on this portion of chromosome 9p exert(s) an effect on esophageal cancer development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6094-6096 |
| Number of pages | 3 |
| Journal | Cancer Research |
| Volume | 54 |
| Issue number | 23 |
| State | Published - Dec 1 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
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Frequent loss of heterozygosity on chromosome 9 in adenocarcinoma and squamous cell carcinoma of the esophagus. / Tarmin, Lila; Yin, Jing; Zhou, Xiaoling; Suzuki, Hiroyuki; Jiang, Hai Yan; Rhyu, Mun Gan; Abraham, John M.; Meltzer, Stephen; Cottrell, John; Meltzer, Stephen J.
In: Cancer Research, Vol. 54, No. 23, 01.12.1994, p. 6094-6096.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Frequent loss of heterozygosity on chromosome 9 in adenocarcinoma and squamous cell carcinoma of the esophagus
AU - Tarmin, Lila
AU - Yin, Jing
AU - Zhou, Xiaoling
AU - Suzuki, Hiroyuki
AU - Jiang, Hai Yan
AU - Rhyu, Mun Gan
AU - Abraham, John M.
AU - Meltzer, Stephen
AU - Cottrell, John
AU - Meltzer, Stephen J.
PY - 1994/12/1
Y1 - 1994/12/1
N2 - Loss of heterozygosity (LOH) affecting chromosome 9p has been shown to occur frequently in head and neck cancer, glioma, mesothelioma, melanoma, lung cancer, and numerous other tumor types. Chromosome 9p is therefore presumed to contain a tumor suppressor gene or genes. Since esophageal cancer shares characteristics with some of the above tumor types, we performed a detailed examination of 60 patients with squamous cell carcinoma or adenocarcinoma of the esophagus for LOH at loci D9S162, IFNA, D9S171, D9S126, D9S104, D9S165, and D9S163. Multiplex polymerase chain reactions were performed with the inclusion of one radiolabeled nucleotide, and products were electrophoresed on denaturing polyacrylamide gels. Thirty-six of the 60 patients (60%) exhibited LOH at one or more loci on chromosome 9p. Eight of 17 patients (47%) with adenocarcinoma manifested LOH, while 28 of 43 (65%) with squamous cell carcinoma showed LOH. LOH was most frequent at loci D9S171 (19 of 23, or 83%) and D9S165 (24 of 32, or 75%). These data support the hypothesis that a tumor suppressor gene or genes located on this portion of chromosome 9p exert(s) an effect on esophageal cancer development.
AB - Loss of heterozygosity (LOH) affecting chromosome 9p has been shown to occur frequently in head and neck cancer, glioma, mesothelioma, melanoma, lung cancer, and numerous other tumor types. Chromosome 9p is therefore presumed to contain a tumor suppressor gene or genes. Since esophageal cancer shares characteristics with some of the above tumor types, we performed a detailed examination of 60 patients with squamous cell carcinoma or adenocarcinoma of the esophagus for LOH at loci D9S162, IFNA, D9S171, D9S126, D9S104, D9S165, and D9S163. Multiplex polymerase chain reactions were performed with the inclusion of one radiolabeled nucleotide, and products were electrophoresed on denaturing polyacrylamide gels. Thirty-six of the 60 patients (60%) exhibited LOH at one or more loci on chromosome 9p. Eight of 17 patients (47%) with adenocarcinoma manifested LOH, while 28 of 43 (65%) with squamous cell carcinoma showed LOH. LOH was most frequent at loci D9S171 (19 of 23, or 83%) and D9S165 (24 of 32, or 75%). These data support the hypothesis that a tumor suppressor gene or genes located on this portion of chromosome 9p exert(s) an effect on esophageal cancer development.
UR - http://www.scopus.com/inward/record.url?scp=0028081164&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028081164&partnerID=8YFLogxK
M3 - Article
C2 - 7954453
AN - SCOPUS:0028081164
VL - 54
SP - 6094
EP - 6096
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0099-7013
IS - 23
ER -