TY - JOUR
T1 - Frequency of indeterminate western blot tests in healthy adults at low risk for human immunodeficiency virus infection
AU - the NIAID AIDS Vaccine Clinical Trials Network
AU - Midthun, Karen
AU - Garrison, Leslie
AU - Clements, Mary Lou
AU - Farzadegan, Homayoon
AU - Fernie, Bruce
AU - Quinn, Thomas
PY - 1990/12
Y1 - 1990/12
N2 - As part of a phase 1 trial of a candidate AIDS vaccine, blood specimens were collected from 168 healthy adult volunteers at minimal or no risk for becoming infected with human immunodeficiency virus type 1 (HIV-1). These specimens were screened for evidence of HIV-1 infection by enzyme immunoassay (EIA) and the Biotech/Du Pont Western blot (n = 168), culture (n = 122), and polymerase chain reaction assay (n = 20). None of the subjects had a positive test result by any of these assays, but 32% had indeterminate Western blot tests, most of which demonstrated a single band of lowintensity. The most common bands were p24 (47%), p55 (34%), and p66 (36%); envelope bands were unusual (gp41, 2%; gp120, 2%). No serum specimen collected after 2–11 months from individuals with indeterminate Western blot results was positive by EIA or Western blot. There was91% agreement in the test results ofthe first and second serum samples when the same lot of Western blot kit was used but only 36% agreement when different lots were used. The Biotech/Du Pont Western blot kit thus frequently yields indeterminate test results in the absence of HIV-1 infection, the reproducibility of which is subject to lot-to-lot variability.
AB - As part of a phase 1 trial of a candidate AIDS vaccine, blood specimens were collected from 168 healthy adult volunteers at minimal or no risk for becoming infected with human immunodeficiency virus type 1 (HIV-1). These specimens were screened for evidence of HIV-1 infection by enzyme immunoassay (EIA) and the Biotech/Du Pont Western blot (n = 168), culture (n = 122), and polymerase chain reaction assay (n = 20). None of the subjects had a positive test result by any of these assays, but 32% had indeterminate Western blot tests, most of which demonstrated a single band of lowintensity. The most common bands were p24 (47%), p55 (34%), and p66 (36%); envelope bands were unusual (gp41, 2%; gp120, 2%). No serum specimen collected after 2–11 months from individuals with indeterminate Western blot results was positive by EIA or Western blot. There was91% agreement in the test results ofthe first and second serum samples when the same lot of Western blot kit was used but only 36% agreement when different lots were used. The Biotech/Du Pont Western blot kit thus frequently yields indeterminate test results in the absence of HIV-1 infection, the reproducibility of which is subject to lot-to-lot variability.
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U2 - 10.1093/infdis/162.6.1379
DO - 10.1093/infdis/162.6.1379
M3 - Article
C2 - 2230270
AN - SCOPUS:0025241413
SN - 0022-1899
VL - 162
SP - 1379
EP - 1382
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 6
ER -