Frequency of EBV LMP-1 promoter and coding variations in burkitt lymphoma samples in Africa and South America and peripheral blood in uganda

Hsiao Mei Liao, Hebing Liu, Heiyan Lei, Bingjie Li, Pei Ju Chin, Shien Tsai, Kishor Bhatia, Marina Gutierrez, Sidnei Epelman, Robert J. Biggar, Francis Nkrumah, Janet Neequaye, Martin D. Ogwang, Steven J. Reynolds, Shyh Ching Lo, Sam M. Mbulaiteye

Research output: Contribution to journalArticlepeer-review

Abstract

Epstein-Barr virus (EBV) is linked to several cancers, including endemic Burkitt lymphoma (eBL), but causal variants are unknown. We recently reported novel sequence variants in the LMP-1 gene and promoter in EBV genomes sequenced from 13 of 14 BL biopsies. Alignments of the novel sequence variants for 114 published EBV genomes, including 27 from BL cases, revealed four LMP-1 variant patterns, designated A to D. Pattern A variant was found in 48% of BL EBV genomes. Here, we used PCR-Sanger sequencing to evaluate 50 additional BL biopsies from Ghana, Brazil, and Argentina, and peripheral blood samples from 113 eBL cases and 115 controls in Uganda. Pattern A was found in 60.9% of 64 BL biopsies evaluated. Compared to PCR-negative subjects in Uganda, detection of Pattern A in peripheral blood was associated with eBL case status (odds ratio [OR] 31.7, 95% confidence interval: 6.8–149), controlling for relevant confounders. Variant Pattern A and Pattern D were associated with eBL case status, but with lower ORs (9.7 and 13.6, respectively). Our results support the hypothesis that EBV LMP-1 Pattern A may be associated with eBL, but it is not the sole associated variant. Further research is needed to replicate and elucidate our findings.

Original languageEnglish (US)
Article number177
JournalCancers
Volume10
Issue number6
DOIs
StatePublished - Jun 2 2018
Externally publishedYes

Keywords

  • Africa
  • Burkitt lymphoma
  • Epstein-Barr virus
  • LMP-1
  • Latin America
  • Next-generation sequencing

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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