Frequency domain lifetimes of liganded and unliganded hemoglobin measured with a 10 GHz instrument using front face geometry on a free liquid surface

Enrico Bucci, Zygmunt Gryczynsky, Clara Fronticelli, Gabor Laczko, Henryk Malak, Joseph R. Lakowicz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present data on lifetimes of hemoglobin solutions obtained using a 10 GHz frequency domain fluorometer and a specially designed cuvette which allows front face excitation on a free liquid surface. The cuvette eliminates reflections and stray emissions, which become significant for low intensity fluorophores like hemoglobin. Three lifetimes are detectable in the subnanosecond range. At high frequency it is possible to detect components below 10 ps. The average lifetime of hemoglobin is ligand dependent, opening a new chapter in the investigation of the allosteric behavior of hemoglobin.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsJoseph F. Lakowicz
PublisherPubl by Int Soc for Optical Engineering
Pages813-819
Number of pages7
ISBN (Print)0819402451
StatePublished - Dec 1 1990
Externally publishedYes
EventTime-Resolved Laser Spectroscopy in Biochemistry II - Los Angeles, CA, USA
Duration: Jan 15 1990Jan 17 1990

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1204 pt 2
ISSN (Print)0277-786X

Other

OtherTime-Resolved Laser Spectroscopy in Biochemistry II
CityLos Angeles, CA, USA
Period1/15/901/17/90

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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