Free radical formation from the antineoplastic agent VP 16-213

J. M S van Maanen, P. R. Kootstra, H. M. Pinedo, C. de Ruiter, J. de Vries

Research output: Contribution to journalArticle

Abstract

Free radical formation from VP 16-213 was studied by ESR spectroscopy. Incubation of VP 16-213 with the one-electron oxidators persulphate-ferrous, myeloperoxidase (MPO)/hydrogen peroxide and horseradish peroxidase (HRP)/hydrogen peroxide readily led to the formation of a free radical. The ESR spectra obtained in the last two cases, were in perfect accord with that of a product obtained by electrochemical oxidation of VP 16-213 at +550 mV. The half-life of the free radical in 1 mM Tris (pH 7.4), 0.1 MNaClat 20°C, was 257 ± 4 s. The signal recorded on incubation with HRP/H2O2 or MPO/H2O2 did not disappear on addition of 0.3 - 1.2 mg/ml microsomal protein. From incubations with rat liver microsomes in the presence of NADPH, no ESR signals were obtained.

Original languageEnglish (US)
Pages (from-to)263-272
Number of pages10
JournalFree Radical Research
Volume1
Issue number4
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Etoposide
Antineoplastic Agents
Free Radicals
Paramagnetic resonance
Horseradish Peroxidase
Hydrogen Peroxide
Peroxidase
Electrochemical oxidation
Liver Microsomes
NADP
Liver
Half-Life
Rats
Spectrum Analysis
Spectroscopy
Electrons
Proteins

Keywords

  • Antineoplastic Agent
  • Electron Spin Resonance
  • Free Radicals

ASJC Scopus subject areas

  • Biochemistry

Cite this

van Maanen, J. M. S., Kootstra, P. R., Pinedo, H. M., de Ruiter, C., & de Vries, J. (1986). Free radical formation from the antineoplastic agent VP 16-213. Free Radical Research, 1(4), 263-272. https://doi.org/10.3109/10715768609051636

Free radical formation from the antineoplastic agent VP 16-213. / van Maanen, J. M S; Kootstra, P. R.; Pinedo, H. M.; de Ruiter, C.; de Vries, J.

In: Free Radical Research, Vol. 1, No. 4, 1986, p. 263-272.

Research output: Contribution to journalArticle

van Maanen, JMS, Kootstra, PR, Pinedo, HM, de Ruiter, C & de Vries, J 1986, 'Free radical formation from the antineoplastic agent VP 16-213', Free Radical Research, vol. 1, no. 4, pp. 263-272. https://doi.org/10.3109/10715768609051636
van Maanen JMS, Kootstra PR, Pinedo HM, de Ruiter C, de Vries J. Free radical formation from the antineoplastic agent VP 16-213. Free Radical Research. 1986;1(4):263-272. https://doi.org/10.3109/10715768609051636
van Maanen, J. M S ; Kootstra, P. R. ; Pinedo, H. M. ; de Ruiter, C. ; de Vries, J. / Free radical formation from the antineoplastic agent VP 16-213. In: Free Radical Research. 1986 ; Vol. 1, No. 4. pp. 263-272.
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