Free radical formation from the antineoplastic agent VP 16-213

J. M S van Maanen; P. R. Kootstra; H. M. Pinedo; C. de Ruiter; J. de Vries

doi:10.3109/10715768609051636

Free radical formation from the antineoplastic agent VP 16-213

J. M S van Maanen, P. R. Kootstra, H. M. Pinedo, C. de Ruiter, J. de Vries

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Free radical formation from VP 16-213 was studied by ESR spectroscopy. Incubation of VP 16-213 with the one-electron oxidators persulphate-ferrous, myeloperoxidase (MPO)/hydrogen peroxide and horseradish peroxidase (HRP)/hydrogen peroxide readily led to the formation of a free radical. The ESR spectra obtained in the last two cases, were in perfect accord with that of a product obtained by electrochemical oxidation of VP 16-213 at +550 mV. The half-life of the free radical in 1 mM Tris (pH 7.4), 0.1 MNaClat 20°C, was 257 ± 4 s. The signal recorded on incubation with HRP/H₂O₂ or MPO/H₂O₂ did not disappear on addition of 0.3 - 1.2 mg/ml microsomal protein. From incubations with rat liver microsomes in the presence of NADPH, no ESR signals were obtained.

Original language	English (US)
Pages (from-to)	263-272
Number of pages	10
Journal	Free Radical Research
Volume	1
Issue number	4
DOIs	https://doi.org/10.3109/10715768609051636
State	Published - 1986
Externally published	Yes

Keywords

Antineoplastic Agent
Electron Spin Resonance
Free Radicals

ASJC Scopus subject areas

Biochemistry

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10.3109/10715768609051636

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@article{589a2cd91a9b4891b810ea4348dff7c2,

title = "Free radical formation from the antineoplastic agent VP 16-213",

abstract = "Free radical formation from VP 16-213 was studied by ESR spectroscopy. Incubation of VP 16-213 with the one-electron oxidators persulphate-ferrous, myeloperoxidase (MPO)/hydrogen peroxide and horseradish peroxidase (HRP)/hydrogen peroxide readily led to the formation of a free radical. The ESR spectra obtained in the last two cases, were in perfect accord with that of a product obtained by electrochemical oxidation of VP 16-213 at +550 mV. The half-life of the free radical in 1 mM Tris (pH 7.4), 0.1 MNaClat 20°C, was 257 ± 4 s. The signal recorded on incubation with HRP/H2O2 or MPO/H2O2 did not disappear on addition of 0.3 - 1.2 mg/ml microsomal protein. From incubations with rat liver microsomes in the presence of NADPH, no ESR signals were obtained.",

keywords = "Antineoplastic Agent, Electron Spin Resonance, Free Radicals",

author = "{van Maanen}, {J. M S} and Kootstra, {P. R.} and Pinedo, {H. M.} and {de Ruiter}, C. and {de Vries}, J.",

year = "1986",

doi = "10.3109/10715768609051636",

language = "English (US)",

volume = "1",

pages = "263--272",

journal = "Free Radical Research",

issn = "1071-5762",

publisher = "Informa Healthcare",

number = "4",

}

TY - JOUR

T1 - Free radical formation from the antineoplastic agent VP 16-213

AU - van Maanen, J. M S

AU - Kootstra, P. R.

AU - Pinedo, H. M.

AU - de Ruiter, C.

AU - de Vries, J.

PY - 1986

Y1 - 1986

N2 - Free radical formation from VP 16-213 was studied by ESR spectroscopy. Incubation of VP 16-213 with the one-electron oxidators persulphate-ferrous, myeloperoxidase (MPO)/hydrogen peroxide and horseradish peroxidase (HRP)/hydrogen peroxide readily led to the formation of a free radical. The ESR spectra obtained in the last two cases, were in perfect accord with that of a product obtained by electrochemical oxidation of VP 16-213 at +550 mV. The half-life of the free radical in 1 mM Tris (pH 7.4), 0.1 MNaClat 20°C, was 257 ± 4 s. The signal recorded on incubation with HRP/H2O2 or MPO/H2O2 did not disappear on addition of 0.3 - 1.2 mg/ml microsomal protein. From incubations with rat liver microsomes in the presence of NADPH, no ESR signals were obtained.

AB - Free radical formation from VP 16-213 was studied by ESR spectroscopy. Incubation of VP 16-213 with the one-electron oxidators persulphate-ferrous, myeloperoxidase (MPO)/hydrogen peroxide and horseradish peroxidase (HRP)/hydrogen peroxide readily led to the formation of a free radical. The ESR spectra obtained in the last two cases, were in perfect accord with that of a product obtained by electrochemical oxidation of VP 16-213 at +550 mV. The half-life of the free radical in 1 mM Tris (pH 7.4), 0.1 MNaClat 20°C, was 257 ± 4 s. The signal recorded on incubation with HRP/H2O2 or MPO/H2O2 did not disappear on addition of 0.3 - 1.2 mg/ml microsomal protein. From incubations with rat liver microsomes in the presence of NADPH, no ESR signals were obtained.

KW - Antineoplastic Agent

KW - Electron Spin Resonance

KW - Free Radicals

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JO - Free Radical Research

JF - Free Radical Research

IS - 4

ER -

Free radical formation from the antineoplastic agent VP 16-213

Abstract

Keywords

ASJC Scopus subject areas

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