Free radical formation from the antineoplastic agent VP 16-213

J. M S van Maanen, P. R. Kootstra, H. M. Pinedo, C. de Ruiter, J. de Vries

Research output: Contribution to journalArticlepeer-review

Abstract

Free radical formation from VP 16-213 was studied by ESR spectroscopy. Incubation of VP 16-213 with the one-electron oxidators persulphate-ferrous, myeloperoxidase (MPO)/hydrogen peroxide and horseradish peroxidase (HRP)/hydrogen peroxide readily led to the formation of a free radical. The ESR spectra obtained in the last two cases, were in perfect accord with that of a product obtained by electrochemical oxidation of VP 16-213 at +550 mV. The half-life of the free radical in 1 mM Tris (pH 7.4), 0.1 MNaClat 20°C, was 257 ± 4 s. The signal recorded on incubation with HRP/H2O2 or MPO/H2O2 did not disappear on addition of 0.3 - 1.2 mg/ml microsomal protein. From incubations with rat liver microsomes in the presence of NADPH, no ESR signals were obtained.

Original languageEnglish (US)
Pages (from-to)263-272
Number of pages10
JournalFree Radical Research
Volume1
Issue number4
DOIs
StatePublished - 1986
Externally publishedYes

Keywords

  • Antineoplastic Agent
  • Electron Spin Resonance
  • Free Radicals

ASJC Scopus subject areas

  • Biochemistry

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