TY - JOUR
T1 - Fragmentation of Sialylated Carbohydrates Using Infrared Atmospheric Pressure MALDI Ion Trap Mass Spectrometry from Cation-Doped Liquid Matrixes
AU - Von Seggern, Christopher E.
AU - Zarek, Paul E.
AU - Cotter, Robert J.
PY - 2003/12/1
Y1 - 2003/12/1
N2 - Infrared atmospheric pressure matrix-assisted laser desorption/ionization on an ion trap mass spectrometer is used to study sialylated oligosaccharides desorbed from the liquid phase. Glycerol doped with various cations provides the opportunity to produce cation-adducted intact molecular ions of sugars. Distinct combinations of cations allow for sialic acid stabilization, as well as differential cleavage, resulting in more complete fragmentation coverage of the oligosaccharide. Alkali and transition metal cations are utilized to create three distinct molecular ion species, involving the adduction of a singly charged cation, two singly charged cations, or a doubly charged cation. From these different molecular ion types, complementary sequence, branching, and linkage information for sialylated oligosaccharides can be deduced.
AB - Infrared atmospheric pressure matrix-assisted laser desorption/ionization on an ion trap mass spectrometer is used to study sialylated oligosaccharides desorbed from the liquid phase. Glycerol doped with various cations provides the opportunity to produce cation-adducted intact molecular ions of sugars. Distinct combinations of cations allow for sialic acid stabilization, as well as differential cleavage, resulting in more complete fragmentation coverage of the oligosaccharide. Alkali and transition metal cations are utilized to create three distinct molecular ion species, involving the adduction of a singly charged cation, two singly charged cations, or a doubly charged cation. From these different molecular ion types, complementary sequence, branching, and linkage information for sialylated oligosaccharides can be deduced.
UR - http://www.scopus.com/inward/record.url?scp=0345168208&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0345168208&partnerID=8YFLogxK
U2 - 10.1021/ac0348367
DO - 10.1021/ac0348367
M3 - Article
C2 - 16465690
AN - SCOPUS:0345168208
SN - 0003-2700
VL - 75
SP - 6523
EP - 6530
JO - Analytical chemistry
JF - Analytical chemistry
IS - 23
ER -