Fragmentation of Sialylated Carbohydrates Using Infrared Atmospheric Pressure MALDI Ion Trap Mass Spectrometry from Cation-Doped Liquid Matrixes

Christopher E. Von Seggern, Paul E. Zarek, Robert J. Cotter

Research output: Contribution to journalArticle

Abstract

Infrared atmospheric pressure matrix-assisted laser desorption/ionization on an ion trap mass spectrometer is used to study sialylated oligosaccharides desorbed from the liquid phase. Glycerol doped with various cations provides the opportunity to produce cation-adducted intact molecular ions of sugars. Distinct combinations of cations allow for sialic acid stabilization, as well as differential cleavage, resulting in more complete fragmentation coverage of the oligosaccharide. Alkali and transition metal cations are utilized to create three distinct molecular ion species, involving the adduction of a singly charged cation, two singly charged cations, or a doubly charged cation. From these different molecular ion types, complementary sequence, branching, and linkage information for sialylated oligosaccharides can be deduced.

Original languageEnglish (US)
Pages (from-to)6523-6530
Number of pages8
JournalAnalytical chemistry
Volume75
Issue number23
DOIs
StatePublished - Dec 1 2003

ASJC Scopus subject areas

  • Analytical Chemistry

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