Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation

Lisa Rooper, Hilal Gurler, Andre A. Kajdacsy-Balla, Maria V. Barbolina

Research output: Contribution to journalArticle

Abstract

Background: The goal of this study was to determine a predominant cell type expressing fractalkine receptor (CX3CR1) in mature ovarian teratomas and to establish functional significance of its expression in cell differentiation. Methods. Specimens of ovarian teratoma and human fetal tissues were analyzed by immunohistochemistry for CX3CR1expression. Ovarian teratocarcinoma cell line PA-1 was used as a model for cell differentiation. Results: We found that the majority of the specimens contained CX 3CR1-positive cells of epidermal lineage. Skin keratinocytes in fetal tissues were also CX3CR1- positive. PA-1 cells with downregulated CX3CR1 failed to express a skin keratinocyte marker cytokeratin 14 when cultured on Matrigel in the presence of a morphogen, bone morphogenic protein 4 (BMP-4), as compared to those expressing scrambled shRNA. Conclusions: Here we demonstrate that CX3CR1 is expressed in both normally (fetal skin) and abnormally (ovarian teratoma) differentiated keratinocytes and is required for cell differentiation into epidermal lineage.

Original languageEnglish (US)
Article number57
JournalJournal of Ovarian Research
Volume6
Issue number1
DOIs
StatePublished - Aug 20 2013

Keywords

  • Cell differentiation
  • Fractalkine receptor
  • Mature ovarian teratoma

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

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