Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation

Lisa Rooper, Hilal Gurler, Andre A. Kajdacsy-Balla, Maria V. Barbolina

Research output: Contribution to journalArticle

Abstract

Background: The goal of this study was to determine a predominant cell type expressing fractalkine receptor (CX3CR1) in mature ovarian teratomas and to establish functional significance of its expression in cell differentiation. Methods. Specimens of ovarian teratoma and human fetal tissues were analyzed by immunohistochemistry for CX3CR1expression. Ovarian teratocarcinoma cell line PA-1 was used as a model for cell differentiation. Results: We found that the majority of the specimens contained CX 3CR1-positive cells of epidermal lineage. Skin keratinocytes in fetal tissues were also CX3CR1- positive. PA-1 cells with downregulated CX3CR1 failed to express a skin keratinocyte marker cytokeratin 14 when cultured on Matrigel in the presence of a morphogen, bone morphogenic protein 4 (BMP-4), as compared to those expressing scrambled shRNA. Conclusions: Here we demonstrate that CX3CR1 is expressed in both normally (fetal skin) and abnormally (ovarian teratoma) differentiated keratinocytes and is required for cell differentiation into epidermal lineage.

Original languageEnglish (US)
Article number57
JournalJournal of Ovarian Research
Volume6
Issue number1
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Teratoma
Keratinocytes
Cell Differentiation
Skin
Fetus
Keratin-14
Teratocarcinoma
Cell Lineage
Small Interfering RNA
Down-Regulation
Immunohistochemistry
Bone and Bones
Cell Line
Ovarian Teratoma
V28 receptor
Proteins

Keywords

  • Cell differentiation
  • Fractalkine receptor
  • Mature ovarian teratoma

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

Cite this

Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation. / Rooper, Lisa; Gurler, Hilal; Kajdacsy-Balla, Andre A.; Barbolina, Maria V.

In: Journal of Ovarian Research, Vol. 6, No. 1, 57, 2013.

Research output: Contribution to journalArticle

@article{4b7acb54a88242f4b611d83211b0cc4f,
title = "Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation",
abstract = "Background: The goal of this study was to determine a predominant cell type expressing fractalkine receptor (CX3CR1) in mature ovarian teratomas and to establish functional significance of its expression in cell differentiation. Methods. Specimens of ovarian teratoma and human fetal tissues were analyzed by immunohistochemistry for CX3CR1expression. Ovarian teratocarcinoma cell line PA-1 was used as a model for cell differentiation. Results: We found that the majority of the specimens contained CX 3CR1-positive cells of epidermal lineage. Skin keratinocytes in fetal tissues were also CX3CR1- positive. PA-1 cells with downregulated CX3CR1 failed to express a skin keratinocyte marker cytokeratin 14 when cultured on Matrigel in the presence of a morphogen, bone morphogenic protein 4 (BMP-4), as compared to those expressing scrambled shRNA. Conclusions: Here we demonstrate that CX3CR1 is expressed in both normally (fetal skin) and abnormally (ovarian teratoma) differentiated keratinocytes and is required for cell differentiation into epidermal lineage.",
keywords = "Cell differentiation, Fractalkine receptor, Mature ovarian teratoma",
author = "Lisa Rooper and Hilal Gurler and Kajdacsy-Balla, {Andre A.} and Barbolina, {Maria V.}",
year = "2013",
doi = "10.1186/1757-2215-6-57",
language = "English (US)",
volume = "6",
journal = "Journal of Ovarian Research",
issn = "1757-2215",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation

AU - Rooper, Lisa

AU - Gurler, Hilal

AU - Kajdacsy-Balla, Andre A.

AU - Barbolina, Maria V.

PY - 2013

Y1 - 2013

N2 - Background: The goal of this study was to determine a predominant cell type expressing fractalkine receptor (CX3CR1) in mature ovarian teratomas and to establish functional significance of its expression in cell differentiation. Methods. Specimens of ovarian teratoma and human fetal tissues were analyzed by immunohistochemistry for CX3CR1expression. Ovarian teratocarcinoma cell line PA-1 was used as a model for cell differentiation. Results: We found that the majority of the specimens contained CX 3CR1-positive cells of epidermal lineage. Skin keratinocytes in fetal tissues were also CX3CR1- positive. PA-1 cells with downregulated CX3CR1 failed to express a skin keratinocyte marker cytokeratin 14 when cultured on Matrigel in the presence of a morphogen, bone morphogenic protein 4 (BMP-4), as compared to those expressing scrambled shRNA. Conclusions: Here we demonstrate that CX3CR1 is expressed in both normally (fetal skin) and abnormally (ovarian teratoma) differentiated keratinocytes and is required for cell differentiation into epidermal lineage.

AB - Background: The goal of this study was to determine a predominant cell type expressing fractalkine receptor (CX3CR1) in mature ovarian teratomas and to establish functional significance of its expression in cell differentiation. Methods. Specimens of ovarian teratoma and human fetal tissues were analyzed by immunohistochemistry for CX3CR1expression. Ovarian teratocarcinoma cell line PA-1 was used as a model for cell differentiation. Results: We found that the majority of the specimens contained CX 3CR1-positive cells of epidermal lineage. Skin keratinocytes in fetal tissues were also CX3CR1- positive. PA-1 cells with downregulated CX3CR1 failed to express a skin keratinocyte marker cytokeratin 14 when cultured on Matrigel in the presence of a morphogen, bone morphogenic protein 4 (BMP-4), as compared to those expressing scrambled shRNA. Conclusions: Here we demonstrate that CX3CR1 is expressed in both normally (fetal skin) and abnormally (ovarian teratoma) differentiated keratinocytes and is required for cell differentiation into epidermal lineage.

KW - Cell differentiation

KW - Fractalkine receptor

KW - Mature ovarian teratoma

UR - http://www.scopus.com/inward/record.url?scp=84881513474&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881513474&partnerID=8YFLogxK

U2 - 10.1186/1757-2215-6-57

DO - 10.1186/1757-2215-6-57

M3 - Article

C2 - 23958497

AN - SCOPUS:84881513474

VL - 6

JO - Journal of Ovarian Research

JF - Journal of Ovarian Research

SN - 1757-2215

IS - 1

M1 - 57

ER -