Fractalkine receptor CX 3CR1 is expressed in epithelial ovarian carcinoma cells and required for motility and adhesion to peritoneal mesothelial cells

Mijung Kim, Lisa Rooper, Jia Xie, Andre A. Kajdacsy-Balla, Maria V. Barbolina

Research output: Contribution to journalArticlepeer-review

Abstract

Epithelial ovarian carcinoma (EOC) is a deadly disease, and little is known about the mechanisms underlying its metastatic progression. Using human specimens and established cell lines, we determined that the G-protein-coupled seven-transmembrane fractalkine receptor (CX 3CR1) is expressed in primary and metastatic ovarian carcinoma cells. Ovarian carcinoma cells robustly migrated toward CX 3CL1, a specific ligand of CX 3CR1, in a CX 3CR1-dependent manner. Silencing of CX 3CR1 reduced migration toward human ovarian carcinoma ascites fluid by approximately 70%. Importantly, adhesion of ovarian carcinoma cells to human peritoneal mesothelial cells was dependent on CX 3CL1/CX 3CR1 signaling. In addition, CX 3CL1 was able to induce cellular proliferation. Together, our data suggest that the fractalkine network may function as a major contributor to the progression of EOC, and further attention to its role in the metastasis of this deadly malignancy is warranted.

Original languageEnglish (US)
Pages (from-to)11-24
Number of pages14
JournalMolecular Cancer Research
Volume10
Issue number1
DOIs
StatePublished - Jan 2012
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Oncology
  • Cancer Research

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