TY - JOUR
T1 - Fractalkine receptor CX 3CR1 is expressed in epithelial ovarian carcinoma cells and required for motility and adhesion to peritoneal mesothelial cells
AU - Kim, Mijung
AU - Rooper, Lisa
AU - Xie, Jia
AU - Kajdacsy-Balla, Andre A.
AU - Barbolina, Maria V.
PY - 2012/1
Y1 - 2012/1
N2 - Epithelial ovarian carcinoma (EOC) is a deadly disease, and little is known about the mechanisms underlying its metastatic progression. Using human specimens and established cell lines, we determined that the G-protein-coupled seven-transmembrane fractalkine receptor (CX 3CR1) is expressed in primary and metastatic ovarian carcinoma cells. Ovarian carcinoma cells robustly migrated toward CX 3CL1, a specific ligand of CX 3CR1, in a CX 3CR1-dependent manner. Silencing of CX 3CR1 reduced migration toward human ovarian carcinoma ascites fluid by approximately 70%. Importantly, adhesion of ovarian carcinoma cells to human peritoneal mesothelial cells was dependent on CX 3CL1/CX 3CR1 signaling. In addition, CX 3CL1 was able to induce cellular proliferation. Together, our data suggest that the fractalkine network may function as a major contributor to the progression of EOC, and further attention to its role in the metastasis of this deadly malignancy is warranted.
AB - Epithelial ovarian carcinoma (EOC) is a deadly disease, and little is known about the mechanisms underlying its metastatic progression. Using human specimens and established cell lines, we determined that the G-protein-coupled seven-transmembrane fractalkine receptor (CX 3CR1) is expressed in primary and metastatic ovarian carcinoma cells. Ovarian carcinoma cells robustly migrated toward CX 3CL1, a specific ligand of CX 3CR1, in a CX 3CR1-dependent manner. Silencing of CX 3CR1 reduced migration toward human ovarian carcinoma ascites fluid by approximately 70%. Importantly, adhesion of ovarian carcinoma cells to human peritoneal mesothelial cells was dependent on CX 3CL1/CX 3CR1 signaling. In addition, CX 3CL1 was able to induce cellular proliferation. Together, our data suggest that the fractalkine network may function as a major contributor to the progression of EOC, and further attention to its role in the metastasis of this deadly malignancy is warranted.
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U2 - 10.1158/1541-7786.MCR-11-0256
DO - 10.1158/1541-7786.MCR-11-0256
M3 - Article
C2 - 22064656
AN - SCOPUS:84862908799
SN - 1541-7786
VL - 10
SP - 11
EP - 24
JO - Molecular Cancer Research
JF - Molecular Cancer Research
IS - 1
ER -