Foxp3 + regulatory T cells promote lung epithelial proliferation

J. R. Mock, Brian Thomas Garibaldi, N. R. Aggarwal, J. Jenkins, N. Limjunyawong, B. D. Singer, E. Chau, R. Rabold, D. C. Files, Venkataramana Sidhaye, Wayne A Mitzner, Elizabeth Marie Wagner, Landon Stuart King, Franco D'Alessio

Research output: Contribution to journalArticle

Abstract

Acute respiratory distress syndrome (ARDS) causes significant morbidity and mortality each year. There is a paucity of information regarding the mechanisms necessary for ARDS resolution. Foxp3+ regulatory T cells (Foxp3+ Treg cells) have been shown to be an important determinant of resolution in an experimental model of lung injury. We demonstrate that intratracheal delivery of endotoxin (lipopolysaccharide) elicits alveolar epithelial damage from which the epithelium undergoes proliferation and repair. Epithelial proliferation coincided with an increase in Foxp3+ Treg cells in the lung during the course of resolution. To dissect the role that Foxp3+ Treg cells exert on epithelial proliferation, we depleted Foxp3+ Treg cells, which led to decreased alveolar epithelial proliferation and delayed lung injury recovery. Furthermore, antibody-mediated blockade of CD103, an integrin, which binds to epithelial expressed E-cadherin decreased Foxp3+ Treg numbers and decreased rates of epithelial proliferation after injury. In a non-inflammatory model of regenerative alveologenesis, left lung pneumonectomy, we found that Foxp3+ Treg cells enhanced epithelial proliferation. Moreover, Foxp3+ Treg cells co-cultured with primary type II alveolar cells (AT2) directly increased AT2 cell proliferation in a CD103-dependent manner. These studies provide evidence of a new and integral role for Foxp3+ Treg cells in repair of the lung epithelium.

Original languageEnglish (US)
Pages (from-to)1440-1451
Number of pages12
JournalMucosal Immunology
Volume7
Issue number6
DOIs
StatePublished - Nov 25 2014

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Regulatory T-Lymphocytes
Lung
Adult Respiratory Distress Syndrome
Lung Injury
Epithelium
Alveolar Epithelial Cells
Pneumonectomy
Cadherins
Endotoxins
Integrins
Lipopolysaccharides
Theoretical Models
Cell Proliferation
Morbidity
Mortality
Antibodies
Wounds and Injuries

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Mock, J. R., Garibaldi, B. T., Aggarwal, N. R., Jenkins, J., Limjunyawong, N., Singer, B. D., ... D'Alessio, F. (2014). Foxp3 + regulatory T cells promote lung epithelial proliferation. Mucosal Immunology, 7(6), 1440-1451. https://doi.org/10.1038/mi.2014.33

Foxp3 + regulatory T cells promote lung epithelial proliferation. / Mock, J. R.; Garibaldi, Brian Thomas; Aggarwal, N. R.; Jenkins, J.; Limjunyawong, N.; Singer, B. D.; Chau, E.; Rabold, R.; Files, D. C.; Sidhaye, Venkataramana; Mitzner, Wayne A; Wagner, Elizabeth Marie; King, Landon Stuart; D'Alessio, Franco.

In: Mucosal Immunology, Vol. 7, No. 6, 25.11.2014, p. 1440-1451.

Research output: Contribution to journalArticle

Mock, JR, Garibaldi, BT, Aggarwal, NR, Jenkins, J, Limjunyawong, N, Singer, BD, Chau, E, Rabold, R, Files, DC, Sidhaye, V, Mitzner, WA, Wagner, EM, King, LS & D'Alessio, F 2014, 'Foxp3 + regulatory T cells promote lung epithelial proliferation', Mucosal Immunology, vol. 7, no. 6, pp. 1440-1451. https://doi.org/10.1038/mi.2014.33
Mock JR, Garibaldi BT, Aggarwal NR, Jenkins J, Limjunyawong N, Singer BD et al. Foxp3 + regulatory T cells promote lung epithelial proliferation. Mucosal Immunology. 2014 Nov 25;7(6):1440-1451. https://doi.org/10.1038/mi.2014.33
Mock, J. R. ; Garibaldi, Brian Thomas ; Aggarwal, N. R. ; Jenkins, J. ; Limjunyawong, N. ; Singer, B. D. ; Chau, E. ; Rabold, R. ; Files, D. C. ; Sidhaye, Venkataramana ; Mitzner, Wayne A ; Wagner, Elizabeth Marie ; King, Landon Stuart ; D'Alessio, Franco. / Foxp3 + regulatory T cells promote lung epithelial proliferation. In: Mucosal Immunology. 2014 ; Vol. 7, No. 6. pp. 1440-1451.
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