Four paralogous protein 4.1 genes map to distinct chromosomes in mouse and human

Luanne L. Peters, Heinz Ulrich G Weier, Loren D. Walensky, Solomon H Snyder, Marilyn Parra, Narla Mohandas, John G. Conboy

Research output: Contribution to journalArticle

Abstract

Four highly conserved members of the skeletal protein 4.1 gene family encode a diverse array of protein isoforms via tissue-specific transcription and developmentally regulated alternative pre-mRNA splicing. In addition to the prototypical red blood cell 4.1R (human gene symbol EPB41,) these include two homologues that are strongly expressed in the brain (4.1N, EPB41L1; and 4.1B, EPB41L3) and another that is widely expressed in many tissues (4.1G, EPB41L2). As part of a study on the structure and evolution of the 4.1 genes in human and mouse, we have now completed the chromosomal mapping of their respective loci by reporting the localization of mouse 4.1N, 4.1G, and 4.1B, as well as human 4.1B. For the mouse 4.1 genes, Southern blot analysis of RFLPs in The Jackson Laboratory BSS interspecific backcross yielded the following assignments: 4.1N (Epb4.1l1,) chromosome 2; 4.1G (Epb4.1l2,) chromosome 10; and 4.1B (Epb4.1l3,) mouse chromosome 17. Human 4.1B was physically mapped to chromosome 18p11 using fluorescence in situ hybridization. All of the mouse genes mapped within or adjacent to regions of conserved synteny with corresponding human chromosomes. We conclude that a set of four paralogous 4.1 genes has been evolutionarily conserved in rodents and primates.

Original languageEnglish (US)
Pages (from-to)348-350
Number of pages3
JournalGenomics
Volume54
Issue number2
DOIs
StatePublished - Dec 1 1998

Fingerprint

Chromosomes
Genes
Proteins
Synteny
Chromosomes, Human, Pair 10
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 2
RNA Precursors
Human Chromosomes
Southern Blotting
Fluorescence In Situ Hybridization
Restriction Fragment Length Polymorphisms
Primates
Rodentia
Protein Isoforms
Erythrocytes
Brain

ASJC Scopus subject areas

  • Genetics

Cite this

Peters, L. L., Weier, H. U. G., Walensky, L. D., Snyder, S. H., Parra, M., Mohandas, N., & Conboy, J. G. (1998). Four paralogous protein 4.1 genes map to distinct chromosomes in mouse and human. Genomics, 54(2), 348-350. https://doi.org/10.1006/geno.1998.5537

Four paralogous protein 4.1 genes map to distinct chromosomes in mouse and human. / Peters, Luanne L.; Weier, Heinz Ulrich G; Walensky, Loren D.; Snyder, Solomon H; Parra, Marilyn; Mohandas, Narla; Conboy, John G.

In: Genomics, Vol. 54, No. 2, 01.12.1998, p. 348-350.

Research output: Contribution to journalArticle

Peters, LL, Weier, HUG, Walensky, LD, Snyder, SH, Parra, M, Mohandas, N & Conboy, JG 1998, 'Four paralogous protein 4.1 genes map to distinct chromosomes in mouse and human', Genomics, vol. 54, no. 2, pp. 348-350. https://doi.org/10.1006/geno.1998.5537
Peters LL, Weier HUG, Walensky LD, Snyder SH, Parra M, Mohandas N et al. Four paralogous protein 4.1 genes map to distinct chromosomes in mouse and human. Genomics. 1998 Dec 1;54(2):348-350. https://doi.org/10.1006/geno.1998.5537
Peters, Luanne L. ; Weier, Heinz Ulrich G ; Walensky, Loren D. ; Snyder, Solomon H ; Parra, Marilyn ; Mohandas, Narla ; Conboy, John G. / Four paralogous protein 4.1 genes map to distinct chromosomes in mouse and human. In: Genomics. 1998 ; Vol. 54, No. 2. pp. 348-350.
@article{2c83b623bd62496e8b31e5499d2194a9,
title = "Four paralogous protein 4.1 genes map to distinct chromosomes in mouse and human",
abstract = "Four highly conserved members of the skeletal protein 4.1 gene family encode a diverse array of protein isoforms via tissue-specific transcription and developmentally regulated alternative pre-mRNA splicing. In addition to the prototypical red blood cell 4.1R (human gene symbol EPB41,) these include two homologues that are strongly expressed in the brain (4.1N, EPB41L1; and 4.1B, EPB41L3) and another that is widely expressed in many tissues (4.1G, EPB41L2). As part of a study on the structure and evolution of the 4.1 genes in human and mouse, we have now completed the chromosomal mapping of their respective loci by reporting the localization of mouse 4.1N, 4.1G, and 4.1B, as well as human 4.1B. For the mouse 4.1 genes, Southern blot analysis of RFLPs in The Jackson Laboratory BSS interspecific backcross yielded the following assignments: 4.1N (Epb4.1l1,) chromosome 2; 4.1G (Epb4.1l2,) chromosome 10; and 4.1B (Epb4.1l3,) mouse chromosome 17. Human 4.1B was physically mapped to chromosome 18p11 using fluorescence in situ hybridization. All of the mouse genes mapped within or adjacent to regions of conserved synteny with corresponding human chromosomes. We conclude that a set of four paralogous 4.1 genes has been evolutionarily conserved in rodents and primates.",
author = "Peters, {Luanne L.} and Weier, {Heinz Ulrich G} and Walensky, {Loren D.} and Snyder, {Solomon H} and Marilyn Parra and Narla Mohandas and Conboy, {John G.}",
year = "1998",
month = "12",
day = "1",
doi = "10.1006/geno.1998.5537",
language = "English (US)",
volume = "54",
pages = "348--350",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Four paralogous protein 4.1 genes map to distinct chromosomes in mouse and human

AU - Peters, Luanne L.

AU - Weier, Heinz Ulrich G

AU - Walensky, Loren D.

AU - Snyder, Solomon H

AU - Parra, Marilyn

AU - Mohandas, Narla

AU - Conboy, John G.

PY - 1998/12/1

Y1 - 1998/12/1

N2 - Four highly conserved members of the skeletal protein 4.1 gene family encode a diverse array of protein isoforms via tissue-specific transcription and developmentally regulated alternative pre-mRNA splicing. In addition to the prototypical red blood cell 4.1R (human gene symbol EPB41,) these include two homologues that are strongly expressed in the brain (4.1N, EPB41L1; and 4.1B, EPB41L3) and another that is widely expressed in many tissues (4.1G, EPB41L2). As part of a study on the structure and evolution of the 4.1 genes in human and mouse, we have now completed the chromosomal mapping of their respective loci by reporting the localization of mouse 4.1N, 4.1G, and 4.1B, as well as human 4.1B. For the mouse 4.1 genes, Southern blot analysis of RFLPs in The Jackson Laboratory BSS interspecific backcross yielded the following assignments: 4.1N (Epb4.1l1,) chromosome 2; 4.1G (Epb4.1l2,) chromosome 10; and 4.1B (Epb4.1l3,) mouse chromosome 17. Human 4.1B was physically mapped to chromosome 18p11 using fluorescence in situ hybridization. All of the mouse genes mapped within or adjacent to regions of conserved synteny with corresponding human chromosomes. We conclude that a set of four paralogous 4.1 genes has been evolutionarily conserved in rodents and primates.

AB - Four highly conserved members of the skeletal protein 4.1 gene family encode a diverse array of protein isoforms via tissue-specific transcription and developmentally regulated alternative pre-mRNA splicing. In addition to the prototypical red blood cell 4.1R (human gene symbol EPB41,) these include two homologues that are strongly expressed in the brain (4.1N, EPB41L1; and 4.1B, EPB41L3) and another that is widely expressed in many tissues (4.1G, EPB41L2). As part of a study on the structure and evolution of the 4.1 genes in human and mouse, we have now completed the chromosomal mapping of their respective loci by reporting the localization of mouse 4.1N, 4.1G, and 4.1B, as well as human 4.1B. For the mouse 4.1 genes, Southern blot analysis of RFLPs in The Jackson Laboratory BSS interspecific backcross yielded the following assignments: 4.1N (Epb4.1l1,) chromosome 2; 4.1G (Epb4.1l2,) chromosome 10; and 4.1B (Epb4.1l3,) mouse chromosome 17. Human 4.1B was physically mapped to chromosome 18p11 using fluorescence in situ hybridization. All of the mouse genes mapped within or adjacent to regions of conserved synteny with corresponding human chromosomes. We conclude that a set of four paralogous 4.1 genes has been evolutionarily conserved in rodents and primates.

UR - http://www.scopus.com/inward/record.url?scp=0032402271&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032402271&partnerID=8YFLogxK

U2 - 10.1006/geno.1998.5537

DO - 10.1006/geno.1998.5537

M3 - Article

VL - 54

SP - 348

EP - 350

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 2

ER -