Forskolin and antidiuretic hormone stimulate a Ca2+-activated K+ channel in cultured kidney cells

S. E. Guggino, B. A. Suarez-Isla, W. B. Guggino, B. Sacktor

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Single channels in the apical cell membrane of primary cultured chick kidney cells were studied using the patch clamp technique. Cell-attached recordings revealed the presence of a 107 ± 6 pS channel that increased fractional open time upon depolarization. Experiments with inside-out excised patches indicated that the channel is K+ selective, Ca2+ activated, and inhibited by Ba2+. The addition of forskolin or antidiuretic hormone (ADH) to the bath during cell-attached recordings caused an increase in the fractional open time of the channel. The activation of a K+ channel by increases in cAMP may be one way in which K+ secretion in the kidney is stimulated by ADH in vivo.

Original languageEnglish (US)
Title of host publicationAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume18
Edition3
StatePublished - 1985
Externally publishedYes

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Calcium-Activated Potassium Channels
Colforsin
Vasopressins
Cultured Cells
Kidney
Patch-Clamp Techniques
Baths
Cell Membrane

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Guggino, S. E., Suarez-Isla, B. A., Guggino, W. B., & Sacktor, B. (1985). Forskolin and antidiuretic hormone stimulate a Ca2+-activated K+ channel in cultured kidney cells. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology (3 ed., Vol. 18)

Forskolin and antidiuretic hormone stimulate a Ca2+-activated K+ channel in cultured kidney cells. / Guggino, S. E.; Suarez-Isla, B. A.; Guggino, W. B.; Sacktor, B.

American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 18 3. ed. 1985.

Research output: Chapter in Book/Report/Conference proceedingChapter

Guggino, SE, Suarez-Isla, BA, Guggino, WB & Sacktor, B 1985, Forskolin and antidiuretic hormone stimulate a Ca2+-activated K+ channel in cultured kidney cells. in American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 3 edn, vol. 18.
Guggino SE, Suarez-Isla BA, Guggino WB, Sacktor B. Forskolin and antidiuretic hormone stimulate a Ca2+-activated K+ channel in cultured kidney cells. In American Journal of Physiology - Renal Fluid and Electrolyte Physiology. 3 ed. Vol. 18. 1985
Guggino, S. E. ; Suarez-Isla, B. A. ; Guggino, W. B. ; Sacktor, B. / Forskolin and antidiuretic hormone stimulate a Ca2+-activated K+ channel in cultured kidney cells. American Journal of Physiology - Renal Fluid and Electrolyte Physiology. Vol. 18 3. ed. 1985.
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