Formation of reactive oxygen species and of 8-hydroxy-2'-deoxyguanosine in DNA in vitro with betel-quid ingredients.

M. Friesen, G. Maru, V. Bussachini, H. Bartsch, U. Nair, J. Nair, R. A. Floyd

Research output: Contribution to journalArticlepeer-review

Abstract

Using a chemiluminescence technique, superoxide anion (O2-.) and H2O2 were shown to be formed in vitro, above pH 9.5, from betel-quid (BQ) ingredients, such as areca-nut extract and catechu. The formation of O2-. was enhanced by Fe2+, Fe3+ and Cu2+ and inhibited by Mn2+. Saliva was found to inhibit both O2-. and H2O2 formation from BQ ingredients. Upon incubation of DNA at alkaline pH with areca-nut extract or catechu, in the presence or absence of Fe3+, 8-hydroxy-2'-deoxyguanosine was formed, as quantified by high-performance liquid chromatography. The data suggest a possible role of reactive oxygen species (ROS) in the etiology of oral cancer in betel quid chewers.

Original languageEnglish (US)
Pages (from-to)417-421
Number of pages5
JournalIARC scientific publications
Issue number89
StatePublished - 1988

ASJC Scopus subject areas

  • Medicine(all)

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