Formation of IgE-binding factors by rat T lymphocytes. II. Mechanisms of selective formation of IgE-potentiating factors by treatment with Bordetella pertussis vaccine

M. Hirashima, J. Yodoi, K. Ishizaka

Research output: Contribution to journalArticle

Abstract

Peripheral blood T lymphocytes of rats treated with Bordetella pertussis vaccine (BP) spontaneously released soluble factors that had affinity for IgE and selectively potentiated the IgE response (IgE-potentiating factors). We speculated that lymphoid cells of BP-treated rats formed 'inducers' of IgE-potentiating factors. Indeed, the serum of BP-treated rats as well as culture filtrates of their peripheral blood mononuclear cells induced normal mesenteric lymph node (MLN) cells to form IgE-potentiating factors in vitro. Analysis of the cellular origin of the inducers of IgE-potentiating factors revealed that both adherent cells and nonadherent cells (lymphocytes) were involved in the induction of the factors. Culture filtrates of peritoneal macrophages or peripheral blood adherent mononuclear cells (monocytes) from BP-treated animals induced normals MLN cells to form IgE-binding factors, but these IgE-binding factors failed to potentiate the IgE response of DNP-OA primed cells. However, when norma MLN cells were incubated with adherent cell-derived culture filtrates together with culture filtrates of peripheral blood lymphocytes, IgE-potentiating factors were formed. Rat IgE also induced normal MLN cells to form IgE-binding factors having no biologic activity, but the same cells formed IgE-potentiating factors if they were incubated with IgE in the presence of culture filtrates of peripheral blood lymphocytes of BP-treated rats. Approximately one-half of the IgE-binding factors induced by IgE alone lacked affinity for lentil lectin, whereas the majority of the factors induced in the presence of the culture filtrates of the lymphocytes bound to the lectin. The results collectively indicate that soluble factors from adherent cells of BP-treated rats induce the formation of IgE-binding factors, whereas those from their lymphocytes enhance the glycosylation of IgE-binding factors and thereby provide the IgE-binding factors with a biologic activity - potentiation of the IgE response. The source of the glycosylation-enhancing factors appears to be a subset of T cells that bear W 3/25 markers. Neither the inducers of IgE-binding factors nor the glycosylation-enhancing factors were obtained from normal cells.

Original languageEnglish (US)
Pages (from-to)1804-1810
Number of pages7
JournalJournal of Immunology
Volume127
Issue number5
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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