Formation of cysts by alveolar type II cells in three-dimensional culture reveals a novel mechanism for epithelial morphogenesis

Wei Yu, Xiaohui Fang, Andrew Ewald, Kit Wong, C. Anthony Hunt, Zena Werb, Michael A. Matthay, Keith Mostov

Research output: Contribution to journalArticle

Abstract

Many organs consist of a hollow cavity surrounded by a monolayer of epithelial cells. Despite their common structure, such organs form by diverse morphogenetic processes. Three-dimensional culture systems have been useful in analyzing the events. Most processes require a combination of cell proliferation and cell death to produce a hollow cavity. Here, we describe a new three-dimensional culture system in which primary human lung alveolar type II cells formed hollow epithelial cysts by a novel process. Individual cells moved, collided, and formed alveolar-like cysts without appreciable proliferation or apoptosis. The alveolar-like cysts consisted of a polarized monolayer of differentiated alveolar type II cells, which secreted surfactant into the central lumen. Blockage of β1 integrin did not alter cell movement or collision, but it greatly reduced adhesion of cells after collision and subsequent formation of alveolar-like cysts. Treatment of preformed alveolar-like cysts with forskolin increased their diameter, possibly due to stimulation of fluid secretion into the lumen. We conclude that epithelial differentiation and cyst formation can occur without appreciable proliferation or apoptosis.

Original languageEnglish (US)
Pages (from-to)1693-1700
Number of pages8
JournalMolecular Biology of the Cell
Volume18
Issue number5
DOIs
StatePublished - May 2007
Externally publishedYes

Fingerprint

Alveolar Epithelial Cells
Morphogenesis
Cysts
Apoptosis
Fluids and Secretions
Colforsin
Cell Adhesion
Surface-Active Agents
Integrins
Cell Movement
Cell Death
Epithelial Cells
Cell Proliferation
Lung

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Formation of cysts by alveolar type II cells in three-dimensional culture reveals a novel mechanism for epithelial morphogenesis. / Yu, Wei; Fang, Xiaohui; Ewald, Andrew; Wong, Kit; Hunt, C. Anthony; Werb, Zena; Matthay, Michael A.; Mostov, Keith.

In: Molecular Biology of the Cell, Vol. 18, No. 5, 05.2007, p. 1693-1700.

Research output: Contribution to journalArticle

Yu, Wei ; Fang, Xiaohui ; Ewald, Andrew ; Wong, Kit ; Hunt, C. Anthony ; Werb, Zena ; Matthay, Michael A. ; Mostov, Keith. / Formation of cysts by alveolar type II cells in three-dimensional culture reveals a novel mechanism for epithelial morphogenesis. In: Molecular Biology of the Cell. 2007 ; Vol. 18, No. 5. pp. 1693-1700.
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