Formation of a bilayer lipid membrane on rigid supports: an approach to BLM-based biosensors

Sensory transduction in living cells is thought to involve a change of electrical parameters at the receptor membrane following specific binding events at the membrane surface. Because of the complexity of the biomembrane structure and the environmental factors associated with it, experimental bilayer lipid membranes (BLMs) have been employed for elucidation of processes at the membrane level. This is because the BLM system can be easily probed by a host of powerful and sensitive electrochemical methods. Further, recent advances in microelectronics and biotechnology suggest that the development of a BLM-based electrochemical biosensor may be possible. This paper describes the use of bilayer lipid membranes on solid substrates for analysis of sensor development problems, with relevance to a possible novel type of biomolecular device. Some electrical parameters of the new structure were measured and compared to usual BLM results. The advantages of the self- assembled structure, together with the measuring system, are discussed in terms of stability and sensitivity.