Formation of a barrier by brain microvessel endothelial cells in culture.

K. Dorovini-Zis, P. D. Bowman, A. L. Betz, Gary William Goldstein

Research output: Contribution to journalArticle

Abstract

Endothelial cells (EC) isolated from bovine brain microvessels produce a continuous monolayer when grown in primary culture. The EC are joined together by tight junctions and contain few pinocytotic vesicles. Horseradish peroxidase (HRP) is unable to penetrate this in vitro barrier system. Exposure of the cells to 1.6 M arabinose produces a reversible separation of the tight junctions with penetration of HRP across the monolayer in a pattern similar to that observed in animals after infusion of hyperosmotic solutions into the carotid artery. The behavior of brain microvascular cells in culture suggest that they retain properties important to the formation of the blood-brain barrier.

Original languageEnglish (US)
Pages (from-to)2521-2522
Number of pages2
JournalFederation proceedings
Volume46
Issue number8
StatePublished - Jun 1987
Externally publishedYes

Fingerprint

Tight Junctions
Horseradish Peroxidase
Microvessels
Endothelial Cells
Cell Culture Techniques
Arabinose
Brain
Blood-Brain Barrier
Carotid Arteries
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Formation of a barrier by brain microvessel endothelial cells in culture. / Dorovini-Zis, K.; Bowman, P. D.; Betz, A. L.; Goldstein, Gary William.

In: Federation proceedings, Vol. 46, No. 8, 06.1987, p. 2521-2522.

Research output: Contribution to journalArticle

Dorovini-Zis, K, Bowman, PD, Betz, AL & Goldstein, GW 1987, 'Formation of a barrier by brain microvessel endothelial cells in culture.', Federation proceedings, vol. 46, no. 8, pp. 2521-2522.
Dorovini-Zis, K. ; Bowman, P. D. ; Betz, A. L. ; Goldstein, Gary William. / Formation of a barrier by brain microvessel endothelial cells in culture. In: Federation proceedings. 1987 ; Vol. 46, No. 8. pp. 2521-2522.
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