Food restriction retards age-related biochemical changes in rat small intestine

P. R. Holt, Todd D. Heller, A. G. Richardson

Research output: Contribution to journalArticle

Abstract

Previous studies have demonstrated that the specific activities of several proximal small intestinal mucosal enzymes fall in the aging rat. This reduction was due to a delay in the full expression of activity of these enzymes during epithelial cell transit from the crypt onto the intestinal villus. We now show in the ad libitum fed Fischer 344 rat that jejunal sucrase, maltase, and alkaline phosphatase specific activities do not fall gradually throughout the life span, but are reduced during senesence. Caloric restriction to 60% of ad libitum intake (DR) abolishes or delays this fall in enzyme activity. Jejunal mucosal immunoprecipitable sucrase-isomaltase (S-I) content also falls with age, but sucrase specific activity per molecule of S-I is less in the older ad libitum fed (approximately 45) than in the DR rats (approximately 60). Jejunal lactase activity falls gradually throughout the life span of ad libitum and DR rats, but lactase activity consistently was higher in DR animals. These observations indicate that DR alters the age-related changes in the activity of several enzymes in the rapidly replicating gut mucosa.

Original languageEnglish (US)
JournalJournals of Gerontology
Volume46
Issue number3
StatePublished - 1991
Externally publishedYes

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Sucrase
Small Intestine
Oligo-1,6-Glucosidase
Lactase
Food
Enzymes
Caloric Restriction
alpha-Glucosidases
Inbred F344 Rats
Alkaline Phosphatase
Mucous Membrane
Epithelial Cells

ASJC Scopus subject areas

  • Aging

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Food restriction retards age-related biochemical changes in rat small intestine. / Holt, P. R.; Heller, Todd D.; Richardson, A. G.

In: Journals of Gerontology, Vol. 46, No. 3, 1991.

Research output: Contribution to journalArticle

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