Following mitochondrial footprints through a long mucosal path to lung cancer

Santanu Dasgupta, Rex C. Yung, William H. Westra, David A Rini, Johann Brandes, David Sidransky

Research output: Contribution to journalArticle

Abstract

Background: Mitochondrial DNA (mtDNA) mutations are reported in different tumors. However, there is no information on the temporal development of the mtDNA mutations/content alteration and their extent in normal and abnormal mucosa continuously exposed to tobacco smoke in lung cancer patients. Methodology: We examined the pattern of mtDNA alteration (mtDNA mutation and content index) in 25 airway mucosal biopsies, corresponding tumors and normal lymph nodes obtained from three patients with primary lung cancers. In addition, we examined the pattern of mtDNA mutation in corresponding tumors and normal lymph nodes obtained from eight other patients with primary lung cancers. The entire 16.5 kb mitochondrial genome was sequenced on Affymetrix Mitochip v2.0 sequencing platform in every sample. To examine mtDNA content index, we performed real-time PCR analysis. Principal Findings: The airway mucosal biopsies obtained from three lung cancer patients were histopathologically negative but exhibited multiple clonal mtDNA mutations detectable in the corresponding tumors. One of the patients was operated twice for the removal of tumor from the right upper and left lower lobe respectively within a span of two years. Both of these tumors exhibited twenty identical mtDNA mutations. MtDNA content increased significantly (P

Original languageEnglish (US)
Article numbere6533
JournalPLoS One
Volume4
Issue number8
DOIs
StatePublished - Aug 6 2009

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lung neoplasms
Mitochondrial DNA
Lung Neoplasms
mitochondrial DNA
Tumors
mutation
Mutation
neoplasms
Neoplasms
Biopsy
lymph nodes
biopsy
Lymph Nodes
Mitochondrial Genome
Tobacco
smoke
Smoke
mucosa
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Following mitochondrial footprints through a long mucosal path to lung cancer. / Dasgupta, Santanu; Yung, Rex C.; Westra, William H.; Rini, David A; Brandes, Johann; Sidransky, David.

In: PLoS One, Vol. 4, No. 8, e6533, 06.08.2009.

Research output: Contribution to journalArticle

Dasgupta, Santanu ; Yung, Rex C. ; Westra, William H. ; Rini, David A ; Brandes, Johann ; Sidransky, David. / Following mitochondrial footprints through a long mucosal path to lung cancer. In: PLoS One. 2009 ; Vol. 4, No. 8.
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