Follicular dendritic cells and the persistence of HIV infectivity: The role of antibodies and Fcγ receptors

Beverly A. Smith-Franklin, Brandon F. Keele, John G. Tew, Suzanne Gartner, Andras K. Szakal, Jacob D. Estes, Tyler C. Thacker, Gregory F. Burton

Research output: Contribution to journalArticle

Abstract

Large quantities of HIV are found trapped on the surface of follicular dendritic cells (FDCs), and virus persists on these cells until they ultimately die. We recently found that FDCs maintain HIV infectivity for long periods in vivo and in vitro. Because FDCs trap Ags (and virus) in the form of immune complexes and are rich in FcγRs, we reasoned that Ab and FcγRs may be required for FDC-mediated maintenance of HIV infectivity. To investigate this hypothesis, HIV immune complexes were formed in vitro and incubated for increasing times with or without FDCs, after which the remaining infectious virus was determined by HIV-p24 production in rescue cultures. FDCs maintained HIV infectivity in vitro in a dose-dependent manner but required the presence of specific Ab for this activity regardless of whether laboratory-adapted or primary X4 and R5 isolates were tested. In addition, Abs against either virally or host-encoded proteins on the virion permitted FDC-mediated maintenance of HIV infectivity. We found that the addition of FDCs to HIV immune complexes at the onset of culture gave optimal maintenance of infectivity. Moreover, blocking FDC-FcγRs or killing the FDCs dramatically reduced their ability to preserve virus infectivity. Finally, FDCs appeared to decrease the spontaneous release of HIV-1 gp120, suggesting that FDC-virus interactions stabilize the virus particle, thus contributing to the maintenance of infectivity. Therefore, optimal maintenance of HIV infectivity requires both Ab against particle-associated determinants and FDC-FcγRs.

Original languageEnglish (US)
Pages (from-to)2408-2414
Number of pages7
JournalJournal of Immunology
Volume168
Issue number5
StatePublished - Mar 1 2002
Externally publishedYes

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Follicular Dendritic Cells
Fc Receptors
HIV
Antibodies
Maintenance
Viruses
Antigen-Antibody Complex
Virion
HIV Core Protein p24
Cell Communication

ASJC Scopus subject areas

  • Immunology

Cite this

Smith-Franklin, B. A., Keele, B. F., Tew, J. G., Gartner, S., Szakal, A. K., Estes, J. D., ... Burton, G. F. (2002). Follicular dendritic cells and the persistence of HIV infectivity: The role of antibodies and Fcγ receptors. Journal of Immunology, 168(5), 2408-2414.

Follicular dendritic cells and the persistence of HIV infectivity : The role of antibodies and Fcγ receptors. / Smith-Franklin, Beverly A.; Keele, Brandon F.; Tew, John G.; Gartner, Suzanne; Szakal, Andras K.; Estes, Jacob D.; Thacker, Tyler C.; Burton, Gregory F.

In: Journal of Immunology, Vol. 168, No. 5, 01.03.2002, p. 2408-2414.

Research output: Contribution to journalArticle

Smith-Franklin, BA, Keele, BF, Tew, JG, Gartner, S, Szakal, AK, Estes, JD, Thacker, TC & Burton, GF 2002, 'Follicular dendritic cells and the persistence of HIV infectivity: The role of antibodies and Fcγ receptors', Journal of Immunology, vol. 168, no. 5, pp. 2408-2414.
Smith-Franklin BA, Keele BF, Tew JG, Gartner S, Szakal AK, Estes JD et al. Follicular dendritic cells and the persistence of HIV infectivity: The role of antibodies and Fcγ receptors. Journal of Immunology. 2002 Mar 1;168(5):2408-2414.
Smith-Franklin, Beverly A. ; Keele, Brandon F. ; Tew, John G. ; Gartner, Suzanne ; Szakal, Andras K. ; Estes, Jacob D. ; Thacker, Tyler C. ; Burton, Gregory F. / Follicular dendritic cells and the persistence of HIV infectivity : The role of antibodies and Fcγ receptors. In: Journal of Immunology. 2002 ; Vol. 168, No. 5. pp. 2408-2414.
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