Focused ion beams in biology

Kedar Narayan, Sriram Subramaniam

Research output: Contribution to journalArticle

Abstract

A quiet revolution is under way in technologies used for nanoscale cellular imaging. Focused ion beams, previously restricted to the materials sciences and semiconductor fields, are rapidly becoming powerful tools for ultrastructural imaging of biological samples. Cell and tissue architecture, as preserved in plastic-embedded resin or in plunge-frozen form, can be investigated in three dimensions by scanning electron microscopy imaging of freshly created surfaces that result from the progressive removal of material using a focused ion beam. The focused ion beam can also be used as a sculpting tool to create specific specimen shapes such as lamellae or needles that can be analyzed further by transmission electron microscopy or by methods that probe chemical composition. Here we provide an in-depth primer to the application of focused ion beams in biology, including a guide to the practical aspects of using the technology, as well as selected examples of its contribution to the generation of new insights into subcellular architecture and mechanisms underlying host-pathogen interactions.

Original languageEnglish (US)
Pages (from-to)1021-1031
Number of pages11
JournalNature Methods
Volume12
Issue number11
DOIs
StatePublished - Nov 1 2015
Externally publishedYes

Fingerprint

Focused ion beams
Ions
Imaging techniques
Technology
Host-Pathogen Interactions
Semiconductors
Pathogens
Materials science
Transmission Electron Microscopy
Needles
Electron Scanning Microscopy
Plastics
Resins
Tissue
Semiconductor materials
Transmission electron microscopy
Scanning electron microscopy
Chemical analysis

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Narayan, K., & Subramaniam, S. (2015). Focused ion beams in biology. Nature Methods, 12(11), 1021-1031. https://doi.org/10.1038/nmeth.3623

Focused ion beams in biology. / Narayan, Kedar; Subramaniam, Sriram.

In: Nature Methods, Vol. 12, No. 11, 01.11.2015, p. 1021-1031.

Research output: Contribution to journalArticle

Narayan, K & Subramaniam, S 2015, 'Focused ion beams in biology', Nature Methods, vol. 12, no. 11, pp. 1021-1031. https://doi.org/10.1038/nmeth.3623
Narayan K, Subramaniam S. Focused ion beams in biology. Nature Methods. 2015 Nov 1;12(11):1021-1031. https://doi.org/10.1038/nmeth.3623
Narayan, Kedar ; Subramaniam, Sriram. / Focused ion beams in biology. In: Nature Methods. 2015 ; Vol. 12, No. 11. pp. 1021-1031.
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