Focal brain ischemia in rat: Acute changes in brain tissue T1reflect acute increase in brain tissue water content

Emmanuel L. Barbier, Lijun Liu, Emmanuelle Grillon, Jean François Payen, Jean François Lebas, Christoph Segebarth, Chantal Rémy

Research output: Contribution to journalArticlepeer-review

Abstract

Several recent studies have reported changes of brain tissue T1 in ischemic models during the first minutes after occlusion of the middle cerebral artery (MCA). In order to assess whether these tissue T1 changes are related to an increase in tissue water content, we performed T1 (7 T) and tissue water content measurements in a rat model (n = 10, Sprague-Dawley) of focal cerebral ischemia (intraluminal occlusion model). The tissue water content was determined using a gravimetric technique. The animals were divided into two groups: an ischemic group, with an effective MCA occlusion (n = 6) and a control group, with animals having undergone sham surgery but no MCA occlusion (n = 4). In the ipsilateral cortex, the tissue water content was 81.1 ± 0.7% at 2 h 15 min following ischemic insult (contralateral value: 79.3 ± 0.5%). Concomitantly, the tissue T1 in the ipsilateral cortex was 2062 ± 60 ms at ischemia onset + 1 h (contralateral 1811 ± 28 ms) and 2100 ± 38 ms at ischemia onset +2 h (contralateral 1807 ± 18 ms). The tissue T1 and tissue water content values measured in the contralateral area do not differ from the values obtained in the control group. A significant T1 increase is observed at ischemia onset + 1 h (+ 14%) and ischemia onset (+2 h) + 16%, together with a significant increase in tissue water content (+2.3%). This suggests that there is an increase in tissue water content concomitant with cell swelling during the first hours of ischemia.

Original languageEnglish (US)
Pages (from-to)499-506
Number of pages8
JournalNMR in biomedicine
Volume18
Issue number8
DOIs
StatePublished - Dec 1 2005

Keywords

  • Animal
  • Cytotoxic
  • Edema
  • Gravimetry
  • Inversion recovery
  • Rat
  • Vasogenic

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Spectroscopy

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