Foam pore size is a critical interface parameter of suction-based wound healing devices

Yvonne I. Heit, Pouya Dastouri, Douglas L. Helm, Giorgio Pietramaggiori, George Younan, Paolo Erba, Stefan Münster, Dennis P. Orgill, Sandra S. Scherer

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Suction-based wound healing devices with open-pore foam interfaces are widely used to treat complex tissue defects. The impact of changes in physicochemical parameters of the wound interfaces has not been investigated. METHODS: Full-thickness wounds in diabetic mice were treated with occlusive dressing or a suction device with a polyurethane foam interface varying in mean pore size diameter. Wound surface deformation on day 2 was measured on fixed tissues. Histologic cross-sections were analyzed for granulation tissue thickness (hematoxylin and eosin), myofibroblast density (α-smooth muscle actin), blood vessel density (platelet endothelial cell adhesion molecule-1), and cell proliferation (Ki67) on day 7. RESULTS: Polyurethane foam-induced wound surface deformation increased with polyurethane foam pore diameter: 15 percent (small pore size), 60 percent (medium pore size), and 150 percent (large pore size). The extent of wound strain correlated with granulation tissue thickness that increased 1.7-fold in small pore size foam-treated wounds, 2.5-fold in medium pore size foam-treated wounds, and 4.9-fold in large pore size foam-treated wounds (p < 0.05) compared with wounds treated with an occlusive dressing. All polyurethane foams increased the number of myofibroblasts over occlusive dressing, with maximal presence in large pore size foam-treated wounds compared with all other groups (p < 0.05). CONCLUSIONS: The pore size of the interface material of suction devices has a significant impact on the wound healing response. Larger pores increased wound surface strain, tissue growth, and transformation of contractile cells. Modification of the pore size is a powerful approach for meeting biological needs of specific wounds.

Original languageEnglish (US)
Pages (from-to)589-597
Number of pages9
JournalPlastic and Reconstructive Surgery
Volume129
Issue number3
DOIs
StatePublished - Mar 1 2012
Externally publishedYes

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Suction
Wound Healing
Equipment and Supplies
Wounds and Injuries
Occlusive Dressings
Myofibroblasts
Granulation Tissue
CD31 Antigens
Hematoxylin
Eosine Yellowish-(YS)
Smooth Muscle
Blood Vessels
Actins
Cell Proliferation

ASJC Scopus subject areas

  • Surgery

Cite this

Heit, Y. I., Dastouri, P., Helm, D. L., Pietramaggiori, G., Younan, G., Erba, P., ... Scherer, S. S. (2012). Foam pore size is a critical interface parameter of suction-based wound healing devices. Plastic and Reconstructive Surgery, 129(3), 589-597. https://doi.org/10.1097/PRS.0b013e3182402c89

Foam pore size is a critical interface parameter of suction-based wound healing devices. / Heit, Yvonne I.; Dastouri, Pouya; Helm, Douglas L.; Pietramaggiori, Giorgio; Younan, George; Erba, Paolo; Münster, Stefan; Orgill, Dennis P.; Scherer, Sandra S.

In: Plastic and Reconstructive Surgery, Vol. 129, No. 3, 01.03.2012, p. 589-597.

Research output: Contribution to journalArticle

Heit, YI, Dastouri, P, Helm, DL, Pietramaggiori, G, Younan, G, Erba, P, Münster, S, Orgill, DP & Scherer, SS 2012, 'Foam pore size is a critical interface parameter of suction-based wound healing devices', Plastic and Reconstructive Surgery, vol. 129, no. 3, pp. 589-597. https://doi.org/10.1097/PRS.0b013e3182402c89
Heit, Yvonne I. ; Dastouri, Pouya ; Helm, Douglas L. ; Pietramaggiori, Giorgio ; Younan, George ; Erba, Paolo ; Münster, Stefan ; Orgill, Dennis P. ; Scherer, Sandra S. / Foam pore size is a critical interface parameter of suction-based wound healing devices. In: Plastic and Reconstructive Surgery. 2012 ; Vol. 129, No. 3. pp. 589-597.
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abstract = "BACKGROUND: Suction-based wound healing devices with open-pore foam interfaces are widely used to treat complex tissue defects. The impact of changes in physicochemical parameters of the wound interfaces has not been investigated. METHODS: Full-thickness wounds in diabetic mice were treated with occlusive dressing or a suction device with a polyurethane foam interface varying in mean pore size diameter. Wound surface deformation on day 2 was measured on fixed tissues. Histologic cross-sections were analyzed for granulation tissue thickness (hematoxylin and eosin), myofibroblast density (α-smooth muscle actin), blood vessel density (platelet endothelial cell adhesion molecule-1), and cell proliferation (Ki67) on day 7. RESULTS: Polyurethane foam-induced wound surface deformation increased with polyurethane foam pore diameter: 15 percent (small pore size), 60 percent (medium pore size), and 150 percent (large pore size). The extent of wound strain correlated with granulation tissue thickness that increased 1.7-fold in small pore size foam-treated wounds, 2.5-fold in medium pore size foam-treated wounds, and 4.9-fold in large pore size foam-treated wounds (p < 0.05) compared with wounds treated with an occlusive dressing. All polyurethane foams increased the number of myofibroblasts over occlusive dressing, with maximal presence in large pore size foam-treated wounds compared with all other groups (p < 0.05). CONCLUSIONS: The pore size of the interface material of suction devices has a significant impact on the wound healing response. Larger pores increased wound surface strain, tissue growth, and transformation of contractile cells. Modification of the pore size is a powerful approach for meeting biological needs of specific wounds.",
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AU - Helm, Douglas L.

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AU - Younan, George

AU - Erba, Paolo

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AU - Orgill, Dennis P.

AU - Scherer, Sandra S.

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N2 - BACKGROUND: Suction-based wound healing devices with open-pore foam interfaces are widely used to treat complex tissue defects. The impact of changes in physicochemical parameters of the wound interfaces has not been investigated. METHODS: Full-thickness wounds in diabetic mice were treated with occlusive dressing or a suction device with a polyurethane foam interface varying in mean pore size diameter. Wound surface deformation on day 2 was measured on fixed tissues. Histologic cross-sections were analyzed for granulation tissue thickness (hematoxylin and eosin), myofibroblast density (α-smooth muscle actin), blood vessel density (platelet endothelial cell adhesion molecule-1), and cell proliferation (Ki67) on day 7. RESULTS: Polyurethane foam-induced wound surface deformation increased with polyurethane foam pore diameter: 15 percent (small pore size), 60 percent (medium pore size), and 150 percent (large pore size). The extent of wound strain correlated with granulation tissue thickness that increased 1.7-fold in small pore size foam-treated wounds, 2.5-fold in medium pore size foam-treated wounds, and 4.9-fold in large pore size foam-treated wounds (p < 0.05) compared with wounds treated with an occlusive dressing. All polyurethane foams increased the number of myofibroblasts over occlusive dressing, with maximal presence in large pore size foam-treated wounds compared with all other groups (p < 0.05). CONCLUSIONS: The pore size of the interface material of suction devices has a significant impact on the wound healing response. Larger pores increased wound surface strain, tissue growth, and transformation of contractile cells. Modification of the pore size is a powerful approach for meeting biological needs of specific wounds.

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