Fluoxetine gradually increases [125I]DOI-labelled 5-HT(2A/2C) receptors in the hypothalamus without changing the levels of G(q)- and G11- proteins

Q. Li; N. A. Muma; G. Battaglia; L. D. Van De Kar

doi:10.1016/S0006-8993(97)00961-X

Fluoxetine gradually increases [¹²⁵I]DOI-labelled 5-HT(2A/2C) receptors in the hypothalamus without changing the levels of G(q)- and G₁₁- proteins

Q. Li, N. A. Muma, G. Battaglia, L. D. Van De Kar

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

The time course of fluoxetine-induced supersensitivity of hypothalamic 5-HT(2A/2C) receptors was examined. Daily injections of fluoxetine (7 or 14 days) significantly increased agonist ([¹²⁵I]DOI)-labeled high-affinity- state 5-HT(2A/2C) receptors in the hypothalamus, but not frontal cortex. No change was observed in the density of [³H]ketanserin-labeled 5-HT(2A) receptors in either brain region. The levels of G(q)- and G₁₁- proteins in the hypothalamus and cortex were not altered by fluoxetine. These results suggest that fluoxetine gradually increases the G-protein coupling of 5- HT(2A/2C) receptors without altering the levels of G(q)- or G₁₁-proteins.

Original language	English (US)
Pages (from-to)	225-228
Number of pages	4
Journal	Brain research
Volume	775
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(97)00961-X
State	Published - Nov 14 1997
Externally published	Yes

Keywords

5-HT
5-HT receptor
Antidepressant
G-protein
Receptor coupling
SSRI
Serotonin

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/S0006-8993(97)00961-X

Cite this

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title = "Fluoxetine gradually increases [125I]DOI-labelled 5-HT(2A/2C) receptors in the hypothalamus without changing the levels of G(q)- and G11- proteins",

abstract = "The time course of fluoxetine-induced supersensitivity of hypothalamic 5-HT(2A/2C) receptors was examined. Daily injections of fluoxetine (7 or 14 days) significantly increased agonist ([125I]DOI)-labeled high-affinity- state 5-HT(2A/2C) receptors in the hypothalamus, but not frontal cortex. No change was observed in the density of [3H]ketanserin-labeled 5-HT(2A) receptors in either brain region. The levels of G(q)- and G11- proteins in the hypothalamus and cortex were not altered by fluoxetine. These results suggest that fluoxetine gradually increases the G-protein coupling of 5- HT(2A/2C) receptors without altering the levels of G(q)- or G11-proteins.",

keywords = "5-HT, 5-HT receptor, Antidepressant, G-protein, Receptor coupling, SSRI, Serotonin",

author = "Q. Li and Muma, {N. A.} and G. Battaglia and {Van De Kar}, {L. D.}",

note = "Funding Information: We thank Dr. Theresa M. Cabrera, Wilfred Pinto, and Francisca Garcia for their excellent technical assistance, and Dr. David R. Manning (Univ. of Pennsylvania) for antisera for G q/11 -proteins. Supported in part by USPHS Grants MH45812, NS34153 (L.D.V.d.K.), DA 07741 (G.B.) and NS30460 (N.A.M.). Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

year = "1997",

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doi = "10.1016/S0006-8993(97)00961-X",

language = "English (US)",

volume = "775",

pages = "225--228",

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AU - Li, Q.

AU - Muma, N. A.

AU - Battaglia, G.

AU - Van De Kar, L. D.

N1 - Funding Information: We thank Dr. Theresa M. Cabrera, Wilfred Pinto, and Francisca Garcia for their excellent technical assistance, and Dr. David R. Manning (Univ. of Pennsylvania) for antisera for G q/11 -proteins. Supported in part by USPHS Grants MH45812, NS34153 (L.D.V.d.K.), DA 07741 (G.B.) and NS30460 (N.A.M.). Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 1997/11/14

Y1 - 1997/11/14

N2 - The time course of fluoxetine-induced supersensitivity of hypothalamic 5-HT(2A/2C) receptors was examined. Daily injections of fluoxetine (7 or 14 days) significantly increased agonist ([125I]DOI)-labeled high-affinity- state 5-HT(2A/2C) receptors in the hypothalamus, but not frontal cortex. No change was observed in the density of [3H]ketanserin-labeled 5-HT(2A) receptors in either brain region. The levels of G(q)- and G11- proteins in the hypothalamus and cortex were not altered by fluoxetine. These results suggest that fluoxetine gradually increases the G-protein coupling of 5- HT(2A/2C) receptors without altering the levels of G(q)- or G11-proteins.

AB - The time course of fluoxetine-induced supersensitivity of hypothalamic 5-HT(2A/2C) receptors was examined. Daily injections of fluoxetine (7 or 14 days) significantly increased agonist ([125I]DOI)-labeled high-affinity- state 5-HT(2A/2C) receptors in the hypothalamus, but not frontal cortex. No change was observed in the density of [3H]ketanserin-labeled 5-HT(2A) receptors in either brain region. The levels of G(q)- and G11- proteins in the hypothalamus and cortex were not altered by fluoxetine. These results suggest that fluoxetine gradually increases the G-protein coupling of 5- HT(2A/2C) receptors without altering the levels of G(q)- or G11-proteins.

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KW - Receptor coupling

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