Fluoroquinolone therapy in Mycobacterium chelonae keratitis after lamellar keratectomy

Melvin A. Sarayba, Neda Shamie, B. J. Reiser, Paula M. Sweet, Mehran Taban, Jordan M. Graff, Anna Kesler-Diaz, Kathryn E. Osann, Peter J. McDonnell

Research output: Contribution to journalArticle

Abstract

Purpose: To characterize a rabbit model of Mycobacterium chelonae keratitis after lamellar keratectomy and assess the effectiveness of fluoroquinolone therapy. Setting: University Laboratory, University of California, Irvine, California, USA. Methods: Twenty-eight New Zealand white rabbits had unilateral lamellar keratectomy with placement of 2.5 × 105 colony-forming units of log-phase M chelonae under each flap. Eyes (7 per group) were randomized and treated with sterile balanced salt solution, gatifloxacin 0.3%, ciprofloxacin 0.3%, or levofloxacin 0.5% 4 times daily. Two masked observers examined all eyes on days 2, 5, and 7 and weekly for 4 weeks. Severity of disease and bacterial culture results were the main outcomes measured. The means and standard deviations were calculated, and differences between the groups were statistically analyzed. Results: All eyes developed clinical disease. At the time the rabbits were killed, eyes treated with balanced salt solution, ciprofloxacin, levofloxacin, and gatifloxacin were culture positive in 6 (85.7%), 7 (100%), 6 (85.7%), and 3 (42.9%) of 7 eyes per group, respectively. Frequency of positive culture and the severity of clinical disease in gatifloxacin-treated eyes were significantly less (P < .05) than in the other groups combined. Conclusions: The rabbit model of M chelonae keratitis was successfully developed in our study. A fourth-generation quinolone (gatifloxacin) showed the best performance among the fluoroquinolones tested in our experimental approach. The fourth-generation fluoroquinolone, gatifloxacin, could be effectively used for the treatment of mycobacterial keratitis.

Original languageEnglish (US)
Pages (from-to)1396-1402
Number of pages7
JournalJournal of cataract and refractive surgery
Volume31
Issue number7
DOIs
StatePublished - Jul 2005

ASJC Scopus subject areas

  • Surgery
  • Ophthalmology
  • Sensory Systems

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    Sarayba, M. A., Shamie, N., Reiser, B. J., Sweet, P. M., Taban, M., Graff, J. M., Kesler-Diaz, A., Osann, K. E., & McDonnell, P. J. (2005). Fluoroquinolone therapy in Mycobacterium chelonae keratitis after lamellar keratectomy. Journal of cataract and refractive surgery, 31(7), 1396-1402. https://doi.org/10.1016/j.jcrs.2004.12.051