Fluorometric assay for measurement of viral neuraminidase—application to the rapid detection of influenza virus in nasal wash specimens

Robert H Yolken, Virginia M. Torsen, Richard Berg, Brian R. Murphy, Y. C. Lee

Research output: Contribution to journalArticle

Abstract

A sensitive assay was developed for the measurement of neuraminidase with use of the 4-methylumbelliferyl-αα-ketoside of N-acetyl-neuraminic acid as a fluorescent substrate. Neuraminidase activity was detected in preparations containing small quantities of cultivated influenza virus as well as in some nasal wash specimens from human volunteers experimentally infected with influenza A/Alaska/78 virus. Activity due to influenza viral neuraminidase could be distinguished from activity due to bacterial or mammalian (fibroblast) neuraminidase by means of enzyme inhibition with ethylenediaminetetra- acetate, a chelating agent. The same substrate could be used in a solid-phase assay to determine the antigenic type of the influenza viral neuraminidase. The fluorescent neuraminidase assay takes 2-4 hr to perform and uses equipment available in many laboratories. Thus, it offers promise for the rapid detection of influenza viruses in cell cultures and clinical specimens.

Original languageEnglish (US)
Pages (from-to)516-523
Number of pages8
JournalJournal of Infectious Diseases
Volume142
Issue number4
DOIs
StatePublished - 1980

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Neuraminidase
Orthomyxoviridae
Nose
Human Influenza
Neuraminic Acids
Chelating Agents
Volunteers
Acetates
Cell Culture Techniques
Fibroblasts
Viruses
Equipment and Supplies
Enzymes

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Fluorometric assay for measurement of viral neuraminidase—application to the rapid detection of influenza virus in nasal wash specimens. / Yolken, Robert H; Torsen, Virginia M.; Berg, Richard; Murphy, Brian R.; Lee, Y. C.

In: Journal of Infectious Diseases, Vol. 142, No. 4, 1980, p. 516-523.

Research output: Contribution to journalArticle

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