Fluorine-19 magnetic resonance angiography of the mouse

Ruud B. van Heeswijk, Yves Pilloud, Ulrich Flögel, Jürg Schwitter, Matthias Stuber

Research output: Contribution to journalArticle

Abstract

Purpose: To implement and characterize a fluorine-19 (19F) magnetic resonance imaging (MRI) technique and to test the hypothesis that the 19F MRI signal in steady state after intravenous injection of a perfluoro-15-crown-5 ether (PCE) emulsion may be exploited for angiography in a pre-clinical in vivo animal study. Materials and Methods: In vitro at 9.4T, the detection limit of the PCE emulsion at a scan time of 10 min/slice was determined, after which the T1 and T2 of PCE in venous blood were measured. Permission from the local animal use committee was obtained for all animal experiments. 12 μl/g of PCE emulsion was intravenously injected in 11 mice. Gradient echo 1H and 19F images were obtained at identical anatomical levels. Signal-to-noise (SNR) and contrast-to-noise (CNR) ratios were determined for 33 vessels in both the 19F and 1H images, which was followed by vessel tracking to determine the vessel conspicuity for both modalities. Results: In vitro, the detection limit was ~400 μM, while the 19F T1 and T2 were 1350±40 and 25±2 ms. The 19F MR angiograms selectively visualized the vasculature (and the liver parenchyma over time) while precisely coregistering with the 1H images. Due to the lower SNR of 19F compared to 1H (17±8 vs. 83±49, p19F CNR was also lower at 15±8 vs. 52±35 (p19F images (66±11 vs. 56±12, p = 0.002). Conclusion: 19F magnetic resonance angiography of intravenously administered perfluorocarbon emulsions is feasible for a selective and exclusive visualization of the vasculature in vivo.

Original languageEnglish (US)
Article numbere42236
JournalPLoS One
Volume7
Issue number7
DOIs
StatePublished - Jul 27 2012
Externally publishedYes

Fingerprint

fluorine
Angiography
Fluorine
Magnetic Resonance Angiography
Magnetic resonance
Emulsions
emulsions
ethers
Noise
Animals
mice
magnetic resonance imaging
Limit of Detection
detection limit
perfluorocarbons
Imaging techniques
Fluorocarbons
animal experimentation
intravenous injection
Intravenous Injections

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

van Heeswijk, R. B., Pilloud, Y., Flögel, U., Schwitter, J., & Stuber, M. (2012). Fluorine-19 magnetic resonance angiography of the mouse. PLoS One, 7(7), [e42236]. https://doi.org/10.1371/journal.pone.0042236

Fluorine-19 magnetic resonance angiography of the mouse. / van Heeswijk, Ruud B.; Pilloud, Yves; Flögel, Ulrich; Schwitter, Jürg; Stuber, Matthias.

In: PLoS One, Vol. 7, No. 7, e42236, 27.07.2012.

Research output: Contribution to journalArticle

van Heeswijk, RB, Pilloud, Y, Flögel, U, Schwitter, J & Stuber, M 2012, 'Fluorine-19 magnetic resonance angiography of the mouse', PLoS One, vol. 7, no. 7, e42236. https://doi.org/10.1371/journal.pone.0042236
van Heeswijk RB, Pilloud Y, Flögel U, Schwitter J, Stuber M. Fluorine-19 magnetic resonance angiography of the mouse. PLoS One. 2012 Jul 27;7(7). e42236. https://doi.org/10.1371/journal.pone.0042236
van Heeswijk, Ruud B. ; Pilloud, Yves ; Flögel, Ulrich ; Schwitter, Jürg ; Stuber, Matthias. / Fluorine-19 magnetic resonance angiography of the mouse. In: PLoS One. 2012 ; Vol. 7, No. 7.
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