Fluorescent protein-based biosensors: resolving spatiotemporal dynamics of signaling

Lisa M. DiPilato, Jin Zhang

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Cellular processes are orchestrated by the precise coordination and regulation of molecular events in the cell. Fluorescent protein-based biosensors coupled with live-cell imaging have enabled the visualization of these events in real time and helped shape some of the current concepts of signal transduction, such as spatial compartmentation. The quantitative information produced by these tools has been incorporated into mathematical models that are capable of predicting highly complex and dynamic behaviors of cellular signaling networks, thus providing a systems level understanding of how pathways interact to produce a functional response. Finally, with technological advances in high-throughput and in vivo imaging, these molecular tools promise to continually engender significant contributions to our understanding of cellular processes under normal and diseased conditions.

Original languageEnglish (US)
Pages (from-to)37-42
Number of pages6
JournalCurrent Opinion in Chemical Biology
Issue number1
StatePublished - Feb 2010

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry


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