Fluorescent probes of α- and β-adrenergic and opiate receptors: Biochemical and histochemical evaluation

F. M A Corrêa, R. B. Innis, B. Rouot, G. W. Pasternak, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Dansyl derivatives of the opiate antagonist naloxazone and the α-adrenergic blocking drug 2-[(2′,6′-dimethoxy)phenoxyethylamino]methyl-benzodioxan (WB-4101) were synthesized as potential in vivo fluorescent labels for opiate and α-adrenergic receptors, respectively. When assayed in vitro dansyl analogs display Ki-values for [3H]naloxone and [3H]WB-4101 binding of 8 nM and 80 nM, respectively. The same patterns of histofluorescence are observed in rat brain slices after the intravenous or intracerebroventricular injection of both dansyl drugs. The same patterns are also observed in dansyl-propranolol and 9-aminoacridine-propranolol (9-AAPN) treated animals as well as in untreated control rats. Accordingly, the observed fluorescence does not reflect the labeling of any receptor but is consistent with the distribution of lipofuscin, an endogenous fluorescent compound.

Original languageEnglish (US)
Pages (from-to)47-53
Number of pages7
JournalNeuroscience Letters
Volume16
Issue number1
DOIs
StatePublished - 1980

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Opioid Receptors
Fluorescent Dyes
Adrenergic Receptors
Opiate Alkaloids
Lipofuscin
Naloxone
Propranolol
Adrenergic Agents
Fluorescence
Injections
Brain
Pharmaceutical Preparations
(2-(2',6'-dimethoxy)phenoxyethylamino)methylbenzo-1,4-dioxane
naloxazone
In Vitro Techniques
9-aminoacridylpropranolol

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Fluorescent probes of α- and β-adrenergic and opiate receptors : Biochemical and histochemical evaluation. / Corrêa, F. M A; Innis, R. B.; Rouot, B.; Pasternak, G. W.; Snyder, Solomon H.

In: Neuroscience Letters, Vol. 16, No. 1, 1980, p. 47-53.

Research output: Contribution to journalArticle

Corrêa, F. M A ; Innis, R. B. ; Rouot, B. ; Pasternak, G. W. ; Snyder, Solomon H. / Fluorescent probes of α- and β-adrenergic and opiate receptors : Biochemical and histochemical evaluation. In: Neuroscience Letters. 1980 ; Vol. 16, No. 1. pp. 47-53.
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