Fluorescent measurement of the intracellular pH during sporulation of Saccharomyces cerevisiae

Juan Carlos Aon, Sonia Cortassa

Research output: Contribution to journalArticle

Abstract

This work reports the intracellular pH (pH(i)) dynamics of Saccharomyces cerevisiae cells in sporulation medium. Cells loaded with the pH-sensitive dye carboxy-seminaphthorhodafluor-1 (C.SNARF-1) exhibited an alkalization of the pH(i) following the extracellular pH during sporulation in the absence of buffer and almost no change in pH(i) or ΔpH when sporulation was carried out in buffered medium. The results indicate that the pH gradient does not appear to be directly involved in the regulation of acetate uptake during sporulation. However, the alkalization of pH(i) by eliciting a decrease in metabolic fluxes could account for a lower demand for acetate.

Original languageEnglish (US)
Pages (from-to)17-23
Number of pages7
JournalFEMS Microbiology Letters
Volume153
Issue number1
DOIs
StatePublished - Aug 1 1997
Externally publishedYes

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Saccharomyces cerevisiae
Acetates
Proton-Motive Force
Buffers
Coloring Agents

Keywords

  • Fluorescent dye
  • Intracellular pH
  • Saccharomyces cerevisiae
  • Sporulation

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Fluorescent measurement of the intracellular pH during sporulation of Saccharomyces cerevisiae. / Aon, Juan Carlos; Cortassa, Sonia.

In: FEMS Microbiology Letters, Vol. 153, No. 1, 01.08.1997, p. 17-23.

Research output: Contribution to journalArticle

Aon, Juan Carlos ; Cortassa, Sonia. / Fluorescent measurement of the intracellular pH during sporulation of Saccharomyces cerevisiae. In: FEMS Microbiology Letters. 1997 ; Vol. 153, No. 1. pp. 17-23.
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