Abstract
The fluorescence spectroscopy of single molecule dynamics and its biological applications were studied. It provided tools for testing semi-classical and quantum mechanical theories of light-matter interaction and molecular interactions. Properties of a single fluorescent probe attached to a macromolecule such as very high sensitivity of the fluorophore to its immediate local environment, including the sensitivity to the presence of other fluorophores and quenchers near-by were studied. Its unique absorption and emission transition dipoles, which could be interrogated by polarized light, were also investigated for providing local structural information.
Original language | English (US) |
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Pages (from-to) | 179-185 |
Number of pages | 7 |
Journal | Proceedings of SPIE - The International Society for Optical Engineering |
Volume | 3273 |
DOIs | |
State | Published - 1998 |
Externally published | Yes |
Event | Laser Techniques for Condensed-Phase and Biological Systems - San Jose, CA, United States Duration: Jan 29 1998 → Jan 31 1998 |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering