TY - JOUR
T1 - Fluorescence quenching
T2 - A tool for single-molecule protein-folding study
AU - Zhuang, Xiaowei
AU - Ha, Taekjip
AU - Kim, Harold D.
AU - Centner, Thomas
AU - Labeit, Siegfried
AU - Chu, Steven
PY - 2000/12/19
Y1 - 2000/12/19
N2 - By using titin as a model system, we have demonstrated that fluorescence quenching can be used to study protein folding at the single molecule level. The unfolded titin molecules with multiple dye molecules attached are able to fold to the native state. In the native folded state, the fluorescence from dye molecules is quenched due to the close proximity between the dye molecules. Unfolding of the titin leads to a dramatic increase in the fluorescence intensity. Such a change makes the folded and unfolded states of a single titin molecule clearly distinguishable and allows us to measure the folding dynamics of individual titin molecules in real time. We have also shown that fluorescence quenching can signal folding and unfolding of a small protein with only one immunoglobulin domain.
AB - By using titin as a model system, we have demonstrated that fluorescence quenching can be used to study protein folding at the single molecule level. The unfolded titin molecules with multiple dye molecules attached are able to fold to the native state. In the native folded state, the fluorescence from dye molecules is quenched due to the close proximity between the dye molecules. Unfolding of the titin leads to a dramatic increase in the fluorescence intensity. Such a change makes the folded and unfolded states of a single titin molecule clearly distinguishable and allows us to measure the folding dynamics of individual titin molecules in real time. We have also shown that fluorescence quenching can signal folding and unfolding of a small protein with only one immunoglobulin domain.
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U2 - 10.1073/pnas.97.26.14241
DO - 10.1073/pnas.97.26.14241
M3 - Article
C2 - 11121030
AN - SCOPUS:0034687786
SN - 0027-8424
VL - 97
SP - 14241
EP - 14244
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 26
ER -