Fluorescence lifetime imaging in turbid media

M. A. O'Leary, D. A. Boas, X. D. Li, B. Chance, A. G. Yodh

Research output: Contribution to journalArticle

Abstract

The lifetime of a fluorophore generally varies in different environments, making the molecule a sensitive indicator of tissue oxygenation, pH, and glucose. However, lifetime measurements are complicated when the fluorophore is embedded in an optically thick, highly scattering medium such as human tissue. We formulate the inverse problem for fluorescence lifetime tomography using diffuse photon density waves, and we demonstrate the technique by deriving spatial images of heterogeneous fluorophore distribution and lifetime, using simulated measurements in heterogeneous turbid media.

Original languageEnglish (US)
Pages (from-to)158-160
Number of pages3
JournalOptics Letters
Volume21
Issue number2
DOIs
StatePublished - Jan 15 1996
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Fingerprint Dive into the research topics of 'Fluorescence lifetime imaging in turbid media'. Together they form a unique fingerprint.

  • Cite this

    O'Leary, M. A., Boas, D. A., Li, X. D., Chance, B., & Yodh, A. G. (1996). Fluorescence lifetime imaging in turbid media. Optics Letters, 21(2), 158-160. https://doi.org/10.1364/OL.21.000158